ver.211004E
SHOWA DENKO K.K. (https://www.shodex.com/)
5
[Guard filter (HK-G): Installation]
(1) At the first time use, connect the flow line to the guard filter inlet alone (do not connect to the analytical
column), set the flow rate at 0.3 mL/min and start the system. Leave it for about 1 minute.
(2) Stop the system and connect the outlet of the guard filter to the inlet of the analytical column, tighten by
hand. Place wrenches on the parts indicated by
★
and tighten additional 30 - 60
o
. After connecting the
guard filter and the analytical column, they can be kept connected together during the storage time.
Attention!
· Tightening too much with wrenches can damages the thread.
· Do not place a wrench on the part indicated by
◎
to tighten or remove the guard filter.
This may loosen the end fitting of the analytical column and results in solvent leak.
[Guard filter (HK-G): Filter replacement]
(1) Place wrenches on the parts indicated by
★
and untighten the connection. Detach the guard filter from
the analytical column. Cap the inlet of the analytical column.
(2) Use wrenches to untighten the guard filter. Replace the old filter with a new filter.
(3) Tighten the holder by hands and then use wrenches to tighten additional 180
o
.
(4) Refer to the steps described in “Guard filter (HK-G): Installation” to connect the guard filter to the analytical
column.
Attention!
· Make sure to use an official replacement filter (product code: F6700100). Product
performance cannot be guaranteed if unofficial filter was used.
7.3 Solvent Exchange
(1) When replacing the solvent, set the column temperature at 40 - 60 °C and start the system at less than
0.3 mL/min. Recommended solvent volume to introduce at each step is 3 to 5 times of the column volume.
(2) Check miscibility/solubility of the desired new solvent and the solvent currently filled in the column.
(3) When replacing the current solvent with a miscible solvent, first introduce 1:1 mixture of the current solvent
and the new solvent, and then replace it with 100 % of the new solvent.
e.g. When replacing THF to DMF, first run 1:1 mixture of THF and DMF and then introduce 100 % DMF.
(4) When replacing the current solvent with a solvent with low miscibility/solubility to the current solvent, first
use a solvent that is miscible/soluble to both eluents, and then replace it with the new solvent.
e.g. When replacing THF to DMF with LiBr, first follow step (3) above, and then replace 100 % DMF to DMF
with LiBr.
Attention!
· Frequent solvent replacement deteriorates the column, and thus not recommended.
