Shodex GPC HK-400 Series Скачать руководство пользователя страница 1

ver.211004E

 

SHOWA DENKO K.K. (https://www.shodex.com/)

 

Operation Manual 

Shodex

 GPC HK-400 series 

 

Please read this manual carefully to achieve the best and consistent column performance for a long time

 

 
 

Important Handling Instructions 

 

 Caution! 

 

 

· Please consult the Safety Data Sheet (SDS) of reagents and solvents used with the column 

and understand their proper handling methods to prevent potential health hazards or death 
from occurring. 

 

· Please wear appropriate personal protective equipment such as lab goggles and gloves 

when handling organic solvents and acid and alkaline reagents. Avoid any direct physical 
contact to prevent chemical injuries.   

 

Before Using the Column 

(1) Please visually inspect the package and outside of the column for any damage.   

 

(2) Please check if product name and serial number written on the column package, column name tag, and     

enclosed Certificate of Analysis (CoA) are matching and correct.   

 

1. Introduction 

Thank you for purchasing the Shodex

 product. Shodex

 GPC HK-400 series is an ultra-rapid analysis type 

size exclusion chromatography column used with organic solvents. The column series is suitable for the 
separations of organic compounds and/or oligomers, and molecular weight distribution analysis of high 
molecular weight polymers. The columns with different exclusion limits are available. Please select columns 
with target molecular weight ranges (see below) that meet the molecular weight range of your samples.

 

 

2. Column Components

 

 
 
 
 
 

 
 
 
 
 
 

 

3. Column Specifications 

 

Product 

Code 

Product Name 

Column Size 

(mm) 

Particle 

Size  

(

μ

m) 

Theoretical 

Plate Number 

(Per Column) 

Target Molecular 

Weight Range

*

 

Exclusion 

Limit

*

 

I.D. Length 

F6025010 

GPC HK-401 

4.6 150  3 

 

 9,000 

100 - 1,500 

   

 

   

2,000 

F6025020 

GPC HK-402

 

4.6 150  3 

 12,000 

200 - 10,000 

       

20,000 

F6025030 

GPC HK-403

 

4.6 150 

 

3.5 

 

 9,000 

2,000 - 70,000 

     

100,000 

F6026040 

GPC HK-404L

 

4.6 150 

 

3.5 

 

 9,000 

100 - 1,000,000 

   

1,000,000 

F6025050 

GPC HK-405

 

4.6 150  3 

 

 7,000 

10,000 - 2,500,000 

   

4,000,000 

F6025060 

GPC HK-406

 

4.6 150 

 

6.5 

 

 5,000 

30,000 - 8,000,000 

 

10,000,000 

 

HK-404L is a mixed-gel column, suitable for the analysis of wide molecular weight ranges. 

 

                                                                                                                                                                       

    *

 Reference value only / Measured with polystyrene 

 

 

Содержание GPC HK-400 Series

Страница 1: ...oduct Shodex GPC HK 400 series is an ultra rapid analysis type size exclusion chromatography column used with organic solvents The column series is suitable for the separations of organic compounds and or oligomers and molecular weight distribution analysis of high molecular weight polymers The columns with different exclusion limits are available Please select columns with target molecular weight...

Страница 2: ...osion when peroxide is concentrated An extra caution should be taken when concentrating the collected sample solution after separation Attention Use the column within above stated flow rate pressure and temperature ranges Using the column outside the given range may damage the column and lower its performance THF without BHT easily forms peroxides and this causes unstable baseline If necessary to ...

Страница 3: ...ent for analysis to dissolve or dilute samples If this is difficult use a solvent which has a composition that is as close as possible to the eluent s composition but which fully dissolves or dilutes the sample 2 Filter the sample solution using disposable 0 45 μm filter to prevent the column from clogging or deteriorating 3 To prepare a sample with molecular weight larger than 1 000 000 first all...

Страница 4: ...e column at an elevated temperature keep a low flow rate until the temperature of the column reaches to the set temperature and then gradually increase the flow rate to the desired 4 Multiple number of columns can be connected in series When connecting multiple columns with different exclusion limits set the column with higher exclusion limit at the upper stream position When connecting HK 404L us...

Страница 5: ...y hands and then use wrenches to tighten additional 180o 4 Refer to the steps described in Guard filter HK G Installation to connect the guard filter to the analytical column Attention Make sure to use an official replacement filter product code F6700100 Product performance cannot be guaranteed if unofficial filter was used 7 3 Solvent Exchange 1 When replacing the solvent set the column temperatu...

Страница 6: ...tainer and not let the solution go through the detector Cleaning Method Insoluble components that block the column inlet may be removed by reversing the flow direction i e introducing the eluent from the column outlet with flow rate at less than half of the recommended flow rate 8 Column Storage Remove the column from the system keeping the solvent used for the analysis Securely tighten the end ca...

Страница 7: ...anyone other than person or firm designated by Showa Denko K K 3 3 If the Shodex product is disposed of 3 4 If the Shodex product is resold by the user without giving prior written notice to Showa Denko K K 3 5 If the performance of the Shodex product is not conform to the specification of the attached CERTIFICATE OF ANALYSIS due to any of the reasons below a Computer virus b Impurities contained ...

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