Shodex Asahipak GS-310 20G Скачать руководство пользователя страница 1

ver.220208E

 

SHOWA DENKO K.K. (https://www.shodex.com/)

 

Operation Manual 

Shodex

 Asahipak

 GS-310 20G, GS-510 20G 

 

Please read this manual carefully to achieve the best and consistent column performance for a long time

 

 
 

Important Handling Instructions 

 

 Caution! 

 

 

· Please consult the Safety Data Sheet (SDS) of reagents and solvents used with the column 

and understand their proper handling methods to prevent potential health hazards or death 
from occurring. 

 

· Please wear appropriate personal protective equipment such as lab goggles and gloves 

when handling organic solvents and acid and alkaline reagents. Avoid any direct physical 
contact to prevent chemical injuries.   

 

Before Using the Column 

(1) Please visually inspect the package and outside of the column for any damage.   

 

(2) Please check if product name and serial number written on the column package, column adhesive label, and     

enclosed Certificate of Analysis (CoA) are matching and correct.   

 

1. Introduction 

Thank you for purchasing the Shodex™ product. Shodex

 Asahipak

 GS-310 20G and GS-510 20G are 

polymer-based size exclusion chromatography columns. They work well with both aqueous and organic solvents.   
 

2. Column Components

                 

 

 
 
 

 
 
 
 
 
 
 
3. Column Specifications 

 

Product 

Code 

Product Name 

Column Size (mm) 

Particle 

Size 

(

μ

m) 

Theoretical 

Plate Number 

(Per Column) 

Target Molecular 

Weight Range

*

 

Exclusion 

Limit

*

 

I.D. Length 

 

F6810038 

Asahipak GS-310 20G

 

20.0 500  13 

 14,000   

300 - 30,000 

  40,000 

F6810039 

Asahipak GS-510 20G

 

20.0 500  13 

 14,000 

5,000 - 200,000 

300,000 

F6710020 

Asahipak GS-10G 7B 

 7.5 

 50 

20 

(Guard Column) 

 

*

 Reference value only / Measured with pullulan 

 

Base Material 

: Spherical porous particles of polyvinyl alcohol

 

Column Housing  : SUS-316 
Screw Type 

: Internally-threaded type No.10-32 UNF 

Shipping Solvent : Water/Methanol = 70/30 

 
 
 
 
 

Содержание Asahipak GS-310 20G

Страница 1: ...check if product name and serial number written on the column package column adhesive label and enclosed Certificate of Analysis CoA are matching and correct 1 Introduction Thank you for purchasing the Shodex product Shodex Asahipak GS 310 20G and GS 510 20G are polymer based size exclusion chromatography columns They work well with both aqueous and organic solvents 2 Column Components 3 Column Sp...

Страница 2: ...ent It is recommended to dedicate the column for this specific use 4 Surfactants such as SDS and Brij 35 can also be added to the eluent However since surfactants tend to remain on the column solvent replacement after their use takes a longer time than replacement from general solvents The replacement time can be shorten by using 30 50 v v methanol Attention Use the column within above stated flow...

Страница 3: ... clogging or deteriorating 3 Recommended injection volume is less than 1 mL per column 4 Viscosity of high molecular weight compound is largely influenced by its molecular weight and concentration Samples with high viscosity cause peak broadening and elution delay and this makes it difficult to obtain their accurate molecular weight distributions In general the larger the molecular weight of the c...

Страница 4: ... at less than 3 0 mL min A rapid increase in pressure can damage the column If the column was used at an elevated temperature lower the flow rate to less than 3 0 mL min at the end of analysis Then turn off the column oven and let the column temperature return to room temperature before stopping the pump If the pump was stopped while the eluent inside the column was still hot as the eluent tempera...

Страница 5: ... column outlet with flow rate at less than half of the recommended flow rate 2 Follow below cleaning steps for adsorbing components For an efficient cleaning reverse the flow direction and use the flow rate at less than 3 0 mL min Introduce 5 to 10 times column volume of the washing solvent Method 1 Adsorption of hydrophilic compounds when using aqueous eluent Introduce the eluent with a high pola...

Страница 6: ...ct on the column Do not drop or hit the column on a hard surface Refer to the Shodex website https www shodex com for product details and their applications For additional assistance contact the distributor from whom you purchased the column or contact your regional Shodex support office https www shodex com en support_office list ...

Страница 7: ...anyone other than person or firm designated by Showa Denko K K 3 3 If the Shodex product is disposed of 3 4 If the Shodex product is resold by the user without giving prior written notice to Showa Denko K K 3 5 If the performance of the Shodex product is not conform to the specification of the attached CERTIFICATE OF ANALYSIS due to any of the reasons below a Computer virus b Impurities contained ...

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