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Bactrox Operations Manual 15
12.3.1
Locate the
“START PASS BOX PURGE” switch which should be in the bottom OFF
position.
NOTE: Do not hold Auto Cycle Start Switch down. Simply press the button and let go to
activate.
12.3.2
Press the
“START PASS BOX PURGE” switch once to initiate the cycle. The PASS
BOX will purge nitrogen thru the PASS BOX for 45 seconds. The
PASS BOX
PURGING
light will alternately flash on and off, indicating the cycle is in progress. When
the cycle is complete, the system will stop automatically and the
PASS BOX PURGING
light will turn off. This light will NOT remain on after a cycle is complete.
12.3.3
If the cycle does not follow the sequences in the previous section on consult the
Troubleshooting Guide for adjustments.
NOTE: Do not hold
“START PASS BOX
PURGE” switch down. Simply Press the button and let go to activate.
12.3.4
If you want to
abort the cycle, press and hold the “START PASS BOX PURGE” switch
for 5 seconds. Then the
“PASS BOX PURGING” pilot light will stop flashing.
13.
ENTERING THE CHAMBER
13.1
The rubber cuffs of the sleeve should be secured around your arm as opposed to clothing.
Remove sharp objects such as rings, watches, bracelets, etc., as they may damage the cuffs.
13.2
Insert your hands and forearms into the sleeves. The cuffs must be firmly secured around your
bare forearms.
13.3
Loosen the arm port door knobs a few turns by turning the black wing nut counterclockwise, and
rotate the locking bars to a horizontal position. The arm port doors can now be pushed forward
into the chamber interior and can be secured on the arm port door holders.
NOTE: Arm movements that are too forceful may cause the sleeves to dislodge from the arm ports.
14.
EXITING THE CHAMBER
14.1
Doors are used to seal the Arm ports while the chamber is not in use. Doors can be stored
using the holders provided while the chamber is in use. Assembly consists of an Arm port door,
tightening knob and locking bar. Doors are secured by turning the locking bars horizontally to
pass through the cutouts in chamber, then turning locking bars vertical and tightening the knobs.
14.2
Before leaving the chamber the incubator doors and the inner PASS BOX door must be closed.
14.3
Hold the locking bars in a horizontal position, and close the doors. Rotate the locking bars to a
vertical position, and gently tighten the black wing nut on both arm port door knobs, just until
they “grab”.
14.4
When the arm port doors are properly sealed, slowly and carefully withdraw both arms from the
sleeves.
15.
MAINTENANCE
15.1
DAILY/ROUTINELY:
Before using any cleaning or decontamination method except those
recommended by the manufacturer, users should check with the manufacturer that the proposed
method will not damage the equipment.
15.2
Remove and/or empty condensate collection container.
15.3
Disinfect the chamber interior. Do not use any volatile cleaning compounds such as alcohol,
bleach or volatile ammonium based cleaners as these compounds will create a toxic
environment for the growth of the microorganisms. Do not use alcohol based cleaning products
on the Plexiglas.
15.4
Check the cuffs on the sleeve system. If the cuffs have holes or any tears, replace.
15.5
Check incubator temperature.
15.6
Record gas tank readings.
15.7
Change/Rejuvenate Anatox. Anatox absorbs volatile fatty acids and hydrogen sulfide in the
chamber. Recommended use is 250 grams placed in a beaker in the chamber.
Anatox-1000 grams (packaged in 250 gram packages)
Instructions
:
Empty two (2) 250 gram packages into two (2) separate 500 ml Pyrex beakers.
Day
one
– place one beaker in the chamber (new Anatox can be placed directly into the
chamber).
