5) Condenser and aperture diaphragm adjustment
a. Condenser up-down
Turn the condenser focusing screw B10 to adjust the
distance between condenser front and the specimen,
and change the equal illumination to obtain the best
brightness.
b. Aperture diaphragm adjustment
Stir the handle of iris aperture of condenser to adjust
size of iris aperture for ajusting the contrast of the
specimen.
6) Centering field diaphragm (this process is for microscope with field
diaphragm)
a. Put 10x objective into light path, turn B5
condenser focusing screw, adjust condenser up or down to get the
image of field diaphragm.
b. Observe throught eyepiece, turn B10 condenser focusing screw, adjust
condenser up or down to get image of field diaphragm.
c. Turn B4 condenser adjusting screw to move the image
to the center of field.
d. Put 40x objective into light path, turn B5 field diaphragm revolver to
make the field diaphragm image bigger than field diaphragm.
Please center it again if the image is not in center.
4.3 Usage of immersion oil objective
The 100x objective of B series microscope can be used for observation even without immersion oil. However,
adding moderate immersion oil between the front lens of 100x objective and the cover glass of specimen can
make the image clearer. Please pay attention that air bubbles and impurities cannot be in the immersion oil,
otherwise, the image would be affected.
First, take 40x objective which has been focused well out from bright path, then take 100x objective into bright
path. At this time, nosepiece or stage moving knob should be turned slightly, and also slightly turn fine focusing
knob to clear away the air bubble of immersion oil, otherwise, the air bubble would affect the image badly.
Fig. 7
Fig. 8
8
Содержание Microlux IV
Страница 1: ...Microlux IV LED Operations Manual...
Страница 16: ......