SE3D r3bEL mini, Руководство пользователя

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r3bEL mini User Manual
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Below is a table of recommended extrusion commands to have a droplet release from the needle
tip for different materials. All testing was done for printing a 3x3 array using a 22-gauge needle
tip.
The parameters being changed are extrusion volume (G1 E### F###), extrusion speed (G1 E###
F###), retraction volume (G1 E-### F###), retraction speed (G1 E-### F###), and wait time
between drops (G4 P####).
Water G1 E1.6 F200
G1 E-0.3 F50
G4 P1000
Alginate (1% w/v) G1 E2.4 F200
G1 E-0.8 F200
G4 P3000
ABTS solution G1 E1.7 F200
G1 E-0.4 F50
G4 P1000
After adjusting your G-code file for the new material or protocol, test it out on the bioprinter and
observe the prints. If you still experience some issues as those described below, follow the
instructions to troubleshoot and modify your protocol.
Drop not releasing from needle tip
This indicates that there isn’t enough liquid is being pushed out of the syringe. This can be fixed
by increasing the E distance in the G1 command.
Two drops releasing from needle tip
This problem can happen for two reasons:
Problem Solution
The previous drop did not release See issue above
The E amount is too high Decrease the E amount in the G1 command
Can’t get consistent droplets even with new values
If this is the case, then it is probably a good idea to adjust the retraction amount. The retraction
command is used to stop the excess flow of liquid after the drop has released. For different
material properties, this value might need to be adjusted. Increase or decrease the negative E
distance in the retraction command to achieve the desired retraction amount.