Description
Item
Description
Item
Sample Loop
10
Mobile Phase
1
Needle
11
Degasser
2
Measuring Pump
12
Pump
3
Low-Pressure Valve
13
Low-Pressure Gradient Unit
4
Autosampler Outlet Tubing
14
LPGE-OUT Tube
5
Column Oven
15
Pump Head
6
Column
16
Mixer (optional)
7
Rinsing Port
17
Autosampler
8
Drain (Mobile Phase Waste
Liquid)
18
High-Pressure Valve
9
Inner Volume of Each Flow Line
The following table describes the inner volume of each flow line for the main unit and the optional flow line parts.
Refer to
Volume (µL)
Flow Line Part Name
Flow Line
Location
275
Pump (Excluding 5 LPGEOUT tube)
3
40
LPGE-OUT tube
4
5
40
Mixer
5,6
7
30
Autosampler (Excluding 10 Sample loop and 14 Autosampler outlet
tubing)
8
230
Sample loop
7
10
4
If a buffer solution is used for the mobile phase, then use LPGE-OUT tube (Long). Derive the system delay volume by replacing 40 μL (LPGEOUT tube
volume), with 315 μL.
Example: 275 + 315 + 40 + 30 + 230 + 35 = 925 μL
5
If trifluoroacetic acid (TFA) fis being used or the mobile phase, then use Mixer MR300 µL (provided with the Delay Volume-Compatible System Kit). Derive
the system delay volume by replacing 40 µL (mixer volume), with 300 µL.
Example: 275 + 40 + 300 + 30 + 230 + 35 = 910 µL
6
If a mixing performance higher than that provided by Mixer MR300 µL is required, then use a mixer with a volume of 780 µL or 2 mL. Replace 40 µL (mixer
volume) with the appropriate volume, 780 µL or 2 mL, to derive the system delay volume.
Example (780 µL): 275+ 40 + 780 + 30 + 230 + 35 = 1390 µL
7
To increase the injection volume of the sample, add Optional Loop (500 µL) or Optional Loop (2 mL) to the sample loop.
When using Optional Loop (500 µL), add 885 µL to 230 µL (sample loop volume), or when using Optional Loop (2 mL), add 3230 µL to derive the system
delay volume.
Example: (Optional Loop [500 µL]): 275 + 40 + 40 + 30 + 230 + 885 + 35 = 1535 µL
Hardware User Guide
ExionLC
™
100 System
RUO-IDV-05-1839-C
28 / 219
Overview
