WARNING! Ionizing Radiation Hazard, Biohazard, or Toxic Chemical
Hazard. Make sure that the electrode protrudes beyond the tip of the
probe, to prevent hazardous vapors from escaping from the source.
The electrode must not be recessed within the probe.
CAUTION: Potential System Damage. If the LC system connected to the mass
spectrometer is not controlled by the software, then do not leave the mass spectrometer
unattended while in operation. The liquid stream from the LC system can flood the ion
source when the mass spectrometer goes in Standby state.
Note:
The minimum flow rate supported by the APCI probe is 200 µL/min. For a complete list of
APCI probe parameters, refer to the section:
Tip!
It is easier to optimize signal and signal-to-noise with flow injection analysis than with
on-column injections.
Note:
When using the APCI probe, make sure that the corona discharge needle is pointing
toward the aperture.
Set Up the System
1. Configure the LC pump to deliver the mobile phase at the required flow rate. Refer to the
section:
Source Parameters and Voltages
.
2. Connect the grounding union on the ion source to an LC pump, through an injector
equipped with a 5 µL loop, or to an autosampler.
3. If an autosampler is being used, then configure the autosampler to perform multiple
injections.
Prepare the System
1. Open the control software.
2. On the Navigation bar, under
Tune and Calibrate
mode, double-click
Manual Tuning
.
3. Open a previously optimized method or create a method based on the compounds.
4. If the ion source has been allowed to cool, then do the following.
a. Set the ion source temperature to
450
.
b. Let the ion source warm up for 30 minutes.
The 30-minute warm-up stage prevents solvent vapors from condensing in the cold probe.
Ion Source Optimization
SCIEX 3200 Systems
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System User Guide
RUO-IDV-05-6475-C
