Focusing
1. Rotate the nosepiece and place the objective lens with the lowest power (4x) in the optical path. Make
sure you hear a click that confirms it is in place.
2. If needed, loosen the locking screw located in between the stage and the arm to regulate the range
of movement of the stage.
3. Ensure the iris diaphragm is opened fully.
4. Push the stage clips to insert a specimen slide.
5. Looking through the eyepiece(s), turn the coarse focuser knob to focus on the specimen until the
specimen comes into focus.
6. If your microscope has a fine focus knob, re-adjust the focus until the image appears sharp and defined.
Changing magnification
Slowly turn the nosepiece clockwise or anticlockwise to the desired magnification until you hear a click
confirming the selected magnification is secured.
1. Ensure your specimen is sitting directly under the objective lens
2. Rotate the coarse and fine focus until the image comes to focus
Using 100X magnification with immersion oil
To obtain maximum resolution with 100X objective lens, applying immersion oil between the slide and
the front lens is necessary.
1. Place one small drop of immersion oil on top of the cover slip, directly over where light comes up
from the condenser.
2. Slowly rotate the 100X objective lens into place. You should see the oil filling the space between the
lens and the slide. If bubbles appear, they can be removed by slightly rotating the revolving nosepiece
back and forth.
3. Adjust the focus with the fine focus knob only.
4. Always wipe and clean all parts that have come into contact with the immersion oil after use. This is
because oil could dry up on the lens surface and damage the microscope. Always wipe with lens paper
or a soft cotton cloth with the appropriate cleaning solution.
Note: The immersion oil should only be used for the 100X objective lens. Do not use the immersion
oil for any other objective lens as this may cause spherical and chromatic aberrations.
saxon
PBM Prodigy Biological Microscope Instruction Manual
