4
5
Instructions for Use Sarpette
®
M
Instructions for Use Sarpette
®
M
When the letter (E) lights up next to the numbers in the display, the selected volume is
no longer in the working range of the pipette. Over-rotating the micrometre screw can
damage the mechanism.
2.3
Tip selection
The SARSTEDT Refill Revolution pipette tips provide the best possible combination of pipette and tip for every
application. Use only tips offered by the manufacturer or compatible with them. Further information and a
detailed brochure can be found at www.refillrevolution.tips.
Reproducibility is improved if each tip is flushed with the material to be pipetted
at least once before use.
2.4
Tip ejection (Figs. 2 and 3)
The patented Justip
TM
system allows immediate positioning of the shaft height (± 2 mm) for an ideal adjustment
and perfect ejection of the tips. The distance between the tip and the ejection rod is set to ~0.5 mm by turning
the ejection screw (< LO – HI >). Built-in click stops prevent unwanted adjustments.
2.5
Protective filter
Macropipettes (5 and 10 ml models) can be provided with a protective filter for additional safety against
penetration of liquid and risk of contamination. This is recommended when dosing large volumes and/or toxic,
radioactive or highly aggressive liquids. It is essential to replace a wet or contaminated filter. The filters are not
autoclavable.
3
Pipetting procedure
Before pipetting, a new, clean tip must be firmly attached.
~ 0.5 mm
1 2 3 4
c
1 2 3 4
c
3.1
Normal pipetting procedure (Fig. 4)
The precisely adjusted volume is aspirated and then discharged.
- Phase 1: Press the operating button until the first stop is reached.
- Phase 2: Immerse the tip vertically approx. 2 – 3 mm deep and slowly release the button. Wait 2
seconds, remove pipette and filled tip vertically without touching the container.
- Phase 3:
Place the tip against the wall of the second container, slowly press the operating button to
the first stop to eject liquid.
- Phase 4:
Press the operating button completely. Residues are expelled from the tip.
Draw the pipette tip 10-15 mm along the wall of the receiver tube.
- Phase 5:
Discard the used tip by pressing the ejection button (C).
3.2
Reverse pipetting procedure
Any excess volume is aspirated, but only the set volume is dispensed.
- Phase 1:
Press the operating button until the overstroke (second stop) is reached.
- Phase 2:
Immerse the tip vertically approx. 2-3 mm deep and slowly release the button. Wait 2 sec.,
remove the pipette and filled tip vertically without touching the container wall.
- Phase 3:
Place the tip on the wall of the second container and press button only up to the first
stop, to deliver the exact set amount of liquid.
- Phase 4:
Remove the pipette from the second container and repeat Phase 2. Expel the remaining
content by pressing the button until the second stop (overstroke). Discard the used tip by
pressing the ejection button (C).
4
Maintenance
As a rule, the models do not require maintenance. Continuous care and cleaning contribute to the optimal
function and longevity of the instrument. It is recommended to check the performance data according to internal
control procedures (SOP, GLP, etc.) or at least once a year. Replace defective parts only with original parts from
the manufacturer.
3
2
1
2
1
3
3
4
flexion sur la tête du piston.
4.1
Checking the seal
Correct functioning of the instrument is only possible if the air cushion closes tightly. Leakage is shown by
droplet formation at the tip or by a different liquid level in the individual tips of a multichannel pipette. In the event
of a leak, we recommend that you have the pipette serviced.
Reverse pipetting
Fig. 5
Forward pipetting
Fig. 4
Fig. 6
Fig. 7
Fig. 8
