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RotaChrom rCPC Operation Manual
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Method creating in the software
Before the method creating the operator prepared and connected the solvents and the dissolved
sample to the rCPC. The gradient box inlet connections are the next:
•
connect the mobile phase to the A line
•
connect the stationary phase to the B line
•
connect the sample to the C line
Firstly, the operator has to write a start method. In the start method filling up the rotor with
stationary phase and set up the detector wavelength. The end of the start method the set up speed
of the rotor has to be the operating speed.
24.
figure: Start method writing
Secondly, the operator has to write a loop method. In the loop method inject the dissolved and
filtered crude with the gradient box C line. Thereafter pumping mobile phase around the same
volume, then the rotor total volume. This called elution. If the main component come down during
the elution, then collect it in the fraction bottles. See in the position column collect fraction from 2
to 10. After the elution pumping stationary phase. It is necessary to refresh the column with fresh
clean solvent and push down the all components from the sample what stayed in the stationary
phase. This called extrusion.
