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OW1.7-VS-CL-LP-640 / USER MANUAL REV1 
November 2017 

2

 

CONTENT 

 

 

CONTENT ................................................................................................................................................. 2

 

1.

 

INTRODUCTION ........................................................................................................................... 3

 

2.

 

SPECIFICATION ............................................................................................................................ 4

 

3. 

 

GETTING STARTED ....................................................................................................................... 6

 

4. 

 

EPIX XCAP .................................................................................................................................... 9

 

5.

 

MICRO-MANAGER ..................................................................................................................... 19

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Содержание OW1.7-VS-CL-LP-640

Страница 1: ...OWL Mini Model OW1 7 VS CL LP 640 USER MANUAL...

Страница 2: ...OW1 7 VS CL LP 640 USER MANUAL REV1 November 2017 2 CONTENT CONTENT 2 1 INTRODUCTION 3 2 SPECIFICATION 4 3 GETTING STARTED 6 4 EPIX XCAP 9 5 MICRO MANAGER 19...

Страница 3: ...ss window between the camera sensor and the microscope or lens Should any other issues occur please contact your local agent To clean the sensor window gently wipe the face of the sensor window with a...

Страница 4: ...ectral response1 0 4 m to 1 7 m Noise RMS 195 electrons Low Gain 176 electrons typical 50 electrons High Gain 40 electrons typical Quantum Efficiency Peak 92 87 1 064nm 82 1 55nm Pixel Well Depth Low...

Страница 5: ...OW1 7 VS CL LP 640 USER MANUAL REV1 November 2017 5 2 2 Mechanical Outline 3D drawings STEP are available upon request...

Страница 6: ...o Microscope The OWL 640 has a standard C Mount that should easily screw onto any microscope port 3 3 Mounting to a tripod or optical table The camera has a 20 BSW Whitworth threaded hole to mount to...

Страница 7: ...on III Eagle Owl 320 Owl 320 High Speed Owl Mini Owl 640 Ninox 640 Owl 1280 In this guide there is a quick overview of XCAP http www epixinc com support files php and Micro Manager https micro manager...

Страница 8: ...light up Boot up the computer Use the Camera Link cable to connect the camera to the computer Connect the other end of the Camera Link cable to the camera Carefully thread the C mount lens onto the c...

Страница 9: ...for the latest cameras and latest PIXCI imaging boards Open the downloaded file when complete and follow the onscreen instructions Be sure to accept the board driver installation 4 2 Operating your ca...

Страница 10: ...reflect your chosen camera For example for the Owl 640 it should read EPIX PIXCI E8DB Raptor Photonics OWL CL 640 If not you need to select a different camera 8 Check that the camera is communicating...

Страница 11: ...Figure 5 Image Capture 12 You should now see an image On the bottom of the screen you should see something similar to the following Frame 0 Size 640 x 512 Resize 0 7x0 7 Video 26 0fps Capture 26 0 fp...

Страница 12: ...window of the EPIX software 4 4 Exposure Click on the Gain Tab There is an option for Auto Level Control ALC When Auto Level Control is unchecked you can manually adjust the exposure and digital gain...

Страница 13: ...igure 8 Trigger Tab 4 6 Auto On this Tab you can select the parameters for the Automatic Level Control Including the speed and spread of signal taken into account In this mode the FPGA of the camera w...

Страница 14: ...s position By default this will be set to account for the whole image Edit the values of Offset Width and Height to place the Auto Level Control ROI over the image Figure 10 Auto ROI Tab Figure 11 Act...

Страница 15: ...the exposure but the box becomes too dark to see any detail This is an extreme example used to illustrate this feature 4 8 NUC On this tab you can select the parameters for the on board Non Uniformit...

Страница 16: ...ber 2017 16 Figure 14 NUC Off Figure 15 NUC On 4 9 TEC Thermoelectric Cooling TEC Use this tab to set and read back the sensor temperature The optimum temperature should be set on start up for the OWL...

Страница 17: ...such that dark areas in the image will appear bright by checking the video invert box Figure 17 Camera Information Output video can be digitally processed to provide image sharpening by checking the...

Страница 18: ...the best display of the RAW image data from the camera 1 Go to the Modify Contrast Modification menu Figure 19 2 Select Stretch Contrast Histogram Percentile Endpoints and Preview Figure 20 Stretch Co...

Страница 19: ...l package available for Windows Mac and Linux it is easy to install and configure right out of the box with built in functionality and user interface for most common tasks performed in a Life Science...

Страница 20: ...ne by going to the downloads section of their website www Micro Manager org You should select the latest nightly build for your operating system found in the Download Nightly Builds section This will...

Страница 21: ...ng Micro Manager for the first time you will see the window shown in Figure 22 You need to make sure none is selected then click Ok Figure 22 Hardware Configuration 1 Start the Hardware Configuration...

Страница 22: ...en look for the camera driver in the RaptorEPIX folder shown in Figure 24 now click Next Figure 24 Add a New Device 3 Continue to click Next until you are faced with the screen shown in Figure 24 4 Re...

Страница 23: ...ember 2017 23 The connected camera should now be recognized Next time you open Micro Manager you can simply select the saved Raptor Owl 640 cfg from the drop down menu figure 26 then click Ok Figure 2...

Страница 24: ...ER MANUAL REV1 November 2017 24 5 3 Operating your camera using Micro Manager Figure 27 Opening Screen 1 Click on snap Figure 27 for Micro Manager to open the viewing window as shown in Figure 28 Figu...

Страница 25: ...Camera settings All of the camera parameters are controlled within the Device Property Browser this can be accessed by selecting Tools from the menu bar then Device Property Browser Figure 29 Camera...

Страница 26: ...7 VS CL LP 640 USER MANUAL REV1 November 2017 26 CORPORATE HEADQUARTERS Raptor Photonics LTD Willowbank Business Park Larne Co Antrim BT40 2SF Northern Ireland PH 44 2828 270141 www raptorphotonics c...

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