PSS Polymer Standards
Service GmbH
In der Dalheimer Wiese 5
55120 Mainz
|
Germany
Polymer Standards
Service-USA, Inc.
160 Old Farm Rd, Suite A
Amherst | MA 01002 | USA
Phone
+49 6131 96239-0
Fax
+49 6131 96239-11
Web
www.pss-polymer.com
Phone
+1 413 835-0265
Fax
+1 413 835-0354
Web
www.pss-polymer.com
Quick Start PSS MCX GPC/SEC Columns
1. Introduction
Thank you for choosing a PSS MCX column. It is a high quality, high performance product ready for
immediate use. This
Quick Start Guide
is designed to get the user up and running quickly with the
common, compatible solvents.
For detailed information on care and use in a wide range of solvents, replacement parts and
accessories, download the
Full User’s Guide
:
www.pss-polymer.com/Resources/manuals/FullUsersGuide_MCX_E.pdf
PSS MCX columns are packed with highly porous particles of a sulfonated styrene-divinylbenzene
copolymer and are supplied as standard in water with 0.75mmol sodium azide. Each column has a
unique serial number and is quality checked for efficiency in the eluent in which the column was
ordered. The test conditions and results are shown on the enclosed certificate of analysis (CoA).
Please keep this in a safe place for future reference.
1.1 Specifications
Column Type
Max. Operating
Pressure
per column
bar / psi
Max. Operating
Pressure for
3 Column Set
bar / psi
Max.
Operating
Temperature
Efficiency
Plates/m
Dextran p1 in
water 0.75mmol
NaN
3
MCX 5µ
60 / 870
120 / 1740
80°C
>40.000
MCX 10µ
40 / 580
80 / 1160
80°C
>20.000
MCX Prep
40 / 580
80 / 1160
80°C
>15.000
1.2 Solvent Compatibility
PSS MCX is compatible with a wide range of aqueous eluents and has optimum performance, when
used with eluents, which have a pH in the range 7.0 to 13.0 for the analysis of sulfonated polyanions.
Transfer to compatible eluents is easy provided the guidelines in this user manual are followed. For
solvents outside this pH range, other PSS column materials may be more appropriate to maintain a
balance between sample ionic character, eluent pH and packing material polarity for a size only based
separation based on the
Magic Triangle
concept. Please visit the
Parameter
Range
Comment
pH Stability
1-13
Chemical stability
Optimum pH range
7-13
Due to anionic nature of the surface
Temperature
<80°C
Operate at 10°-35°C for optimum column lifetime
Organic modifiers
<100% v/v
e.g Methanol, Acetonitrile, Tetrahydrofuran
Salts
< 0.5M
e.g.NaN
3
, NaCl, NaNO
3
Buffers
Compatible with most commonly used buffers
2. Column Installation
When using the column for the first time it is recommended to use the eluent that the column was
originally supplied in and to perform a system plate count noting the backpressure. However, this is
not essential.
2.1 Preparing the GPC/SEC system
Remove any existing columns and store according to the manufacturer’s instructions. Connect the
injector directly to the detector with appropriate tubing and connectors. Transfer the GPC/SEC system
from the current eluent to that required, paying attention to the miscibility and compatibility with the
hardware as per the manufacturer’s instructions.
