1
USER MANUAL
BlotCycler
™
automated western blot processor.
Revision 10.0.2., 02-2014 software ver 3.1
Страница 1: ...1 USER MANUAL BlotCycler automated western blot processor Revision 10 0 2 02 2014 software ver 3 1...
Страница 2: ...ifications and Safety 3 Unpacking and Testing 5 Overview 7 Programming 8 Instrument Set up and Operation 11 Appendix 16 Troubleshooting 17 Fuse replacement 18 Packing Instruction 19 Accessory Products...
Страница 3: ...rator For technical assistance call write fax or email Call 888 490 4443 x 2 Fax 617 812 2672 Email customersupport precisionbiosystems com Write Customer Support Precision Biosystems 241 Francis Aven...
Страница 4: ...arly to 50 from 31 C to 42 C 3 Do not install the equipment near a heating element 4 Do not install the equipment at a place where it may be exposed to corrosive gas 5 Do not install the instrument in...
Страница 5: ...and save them in case you need to send instrument back for service Packing List BlotCycler with six trays Waste tubing attached to unit Power cord Tray lids 2 Tank lid 1 Tray plugs 4 Transformer 220 2...
Страница 6: ...d in the waste solution Left side cleaning Fill water 3 4 cm above minimal level about 2 L make sure water is not leaking Press button and then press button select left side Now you started the cleani...
Страница 7: ...tion vials Pump Antibody container Overview BlotCycler consists of programming display four to six trays for blots trays 1 3 on the left side and trays 4 6 on the right side tank with container for pr...
Страница 8: ...r Interface overview User interface contains 4 buttons press this button to select existing or set up a new protocol press this button to start a protocol press this button to check the status of the...
Страница 9: ...otCycler protocol set up To start programming the operator needs to initialize the system by turning the system OFF and ON using the ON OFF switch on the back of the unit Please wait 3 sec before turn...
Страница 10: ...after blocking PA primary Ab incubation time and SA secondary Ab incubation time Note you can skip washing after blocking by selecting zero but you cannot skip washing after primary and secondary ant...
Страница 11: ...place membranes in the trays Add blocking buffers to each tray containing a membrane Close the trays by replacing the covers Note tray cover has a cut that should be on the upper side to ensure trays...
Страница 12: ...owing screen Press corresponding start button to start cycling for left Tray 1 3 or right side Tray 4 6 A new screen will appear that show the status of current protocol Red blinking dot indicates the...
Страница 13: ...rotocol press button for left or right side Note protocol will start from next step if it was close to the end of the previous step Use to stop shaking while loading blot into trays Protocol on other...
Страница 14: ...ough cleaning solution otherwise the pumps can be damaged Go to the home screen and press button a new screen will appear Press and select left or right side on pop up window Note you can start anothe...
Страница 15: ...is not in operation Go to home screen and press button and then button When all valves are open the beep sound and the message will appear Now you can turn BlotCycler off Note before opening valves ch...
Страница 16: ...Use appropriate transfer times After blotting stain membrane to measure transfer efficiency Protein of interest ran off the gel Use positive control and or molecular weight marker to match gel separat...
Страница 17: ...and purified water to prepare solutions Wear clean gloves at all times Use forceps when handling membranes Run cleaning protocol with cleaning buffer increase the concentration of cleaning buffer two...
Страница 18: ...fuse compartment located on the power entry block using a small flat blade screwdriver or fingernail to gently open the fuse compartment 3 Pull the fuse holder out of the compartment and inspect the...
Страница 19: ...umps into the tank If you cannot find T bar use any appropriate material to secure pump inside tank There are two green Styrofoam covers front and back they are slightly different Back Front Remove tr...
Страница 20: ...n the box Place insert flat box over instrument if you cannot find flat box use any soft material to fill the gap between instrument and box top surface Close and tape the box Ship to Precision Biosys...
Страница 21: ...idization buffer 500 ml BW1000 Washing buffer 10x 1L BW4000 Washing buffer 10x 4L TR1003RL Pair of Block of mini trays right left TR2003L Replacement Block of midi trays left TR2003R Replacement Block...
Страница 22: ...g defects are specifically excluded 1 Defects caused by improper operation and maintenance 2 Repair or modification done by anyone other than Precision Biosystems or their authorized agent 3 Use with...
Страница 23: ...information or technical assistance call write fax or email Call 888 490 4443 x 2 Fax 617 812 2672 Email customersupport precisionbiosystems com Write Customer Support Precision Biosystems 241 Francis...
