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Do  not  handle  specimen  stubs
with  your  bare  fingers.
Contaminates  from  your  fingers
will  volatilize  in  the  high
vacuum  and  contaminate  the
final  beam  aperture  and
detectors  which  in  turn  will
significantly  degrade  the  image.

The  “X”  and  “Y”  controls  are  approximately  centered  at  500  X  500  with  TILT
at  0  degrees.

Do  not  adjust  the  TILT  less  than  0  degrees  nor  greater  than  90  degrees.

Turning  ON  the  electron  beam:

Turn  ON  the  high  tension.    There  should  be  a  “blip”  in  the  filament  current
gauge.    Adjust  the  current  to  saturation  (usually  5-6  clicks).   

Do  not  over-

saturate  the  filament

.

Aligning  the  electron  gun  (if  necessary):

With  a  spot  size  of  500  and  minimum  magnification,  turn  up  the  filament
current  until  the  current  no  longer  rises  on  the  gauge.    Adjust  the  detector
gain  to  provide  a  circular  illumination.    Center  this  circle  on  the  screen
using  the  “gun  shift”  then  use  the  “gun  tilt”  controls  to  give  the  brightest
illumination.

Changing  or  inserting  a  new  specimen:

Be  sure  the  filament  current  and  high  tension  is  off  and  the  filament  has
cooled  for  at  least  1  minute.

Press  the  OFF  button  on  the  vacuum  control  panel.

Press  the  air  button.

Wait  for  the  specimen  chamber  door  to  release  and  then  carefully  pull  the
stage  assembly  out.

Use  the  1.5  mm  Allen  wrench  to  secure
the  specimen  stub  to  the  holder.    Do  not
over-tighten  the  set  screw.    It  only  needs
to  be  firm.

Push  the  stage  assembly  into  the
chamber  being 

absolutely

  sure  that  t h e

specimen  will  not  hit  the  EDAX  detector.

Содержание SEM 525

Страница 1: ...E and adjust brightness and contrast for the display screens once set this does not change appreciably over time If using the Polaroid camera press and hold the photo start button and press the scan rate 2000 and 32 buttons This sets the scan rate for the camera screen The photo scan can be stopped by pressing the interrupt button Adjust operating parameters appropriate to the specimen characteris...

Страница 2: ...isions the following should be expected at filament saturation Bias 1 25 kv saturated filament current 40 µA Bias 2 10 kv saturated filament current 35 µA To lower the saturated filament current increase the filament to Wehnelt distance Select spot size Generally speaking use a large spot size 200 or 500 for low magnification work under 1000 x magnification Smaller spot size is used for higher mag...

Страница 3: ... 500 and minimum magnification turn up the filament current until the current no longer rises on the gauge Adjust the detector gain to provide a circular illumination Center this circle on the screen using the gun shift then use the gun tilt controls to give the brightest illumination Changing or inserting a new specimen Be sure the filament current and high tension is off and the filament has coo...

Страница 4: ...magnification control to maximum lowest lens current setting Turn off the light column switch Turn off the marker switch The SEM can be left in this setting as standby mode for the day Do not leave the SEM on overnight To completely shut down the SEM overnight or longer Press the vacuum OFF button Press the power OFF button Set the cooling water timer to at least 30 minutes to cool the diffusion p...

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