PerkinElmer Wallac 1420, Руководство пользователя

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Tools menu
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Preparing the plate
The wizard explains what samples you should put in the plate and where you should put
them. The strip to be used must be horizontal e.g. A1 to A12 etc. The plate used is the one
defined in the protocol you previously selected.
Blanks should be in A1 and A2; these are used to correct for the plate background in the
acceptor window. These are made by dispensing buffer only.
Spectral crosstalk samples (i.e. donor only samples) in A3 and A4; these are used to
calculate the amount of the blank corrected donor signal in the acceptor window as a
proportion of the donor signal. Only donor and buffer should be dispensed to these wells.
High intensity samples in A5 and A6; these are to normalize the final result to the maximum
donor value. The ratio of the high intensity donor value to the sample donor value corrects
for possible quenching in the sample. These samples should include all the reagents to give
the maximum acceptor signal.
All other wells should be left empty. Then load the plate and click Next .