Pco Edge 3.1, Руководство пользователя

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A6 LIGHTSHEET SCANNING MODE
The pco.edge ‘lightsheet scanning mode’ is a
special mode for lightsheet microscopy and only
available for Camera Link versions
via SDK (not in
pco.camware).
It is based on Rolling Shutter mode and uses the
readout mode
single top down (Mode E see
7.2 ).
Standard line time (see 1.2 ) value is 40 µs 2 and it
can be set from camera specific line time (see
1.2
)
up to 100 ms. An exposure area (between the
orange bars, e.g. five lines 3 ) can be user defined.
It is possible for the user to select the number of
lines and the exposure time per line.
This user-defined capability makes it possible to
synchronize the pco.edge cameras with a
lightsheet microscope that requires this method of
camera exposure timing
Workflow (only PCO SDK)
• Set Rolling Shutter mode
•
Set readout format E
•
Set line timing on (0x0001 ≙ a CMOS_LINETIMING_PARAM_ON
see sc2.defs.h) and set the appropriate line time
•
Set number of exposure lines
•
Set number of delay lines
• Trigger mode and acquire mode and HWIO settings are freely
selectable
SDK settings: (see SDK manual)
SDK function Parameter Value
SC2_SDK_FUNC int WINAPI
PCO_SetCmosLineTiming
wParameter
CMOS_LINETIMING_PA
RM_ON
wTimeBase
TIMEBASE_MS
dwLineTime
e.g. 40
SC2_SDK_FUNC int WINAPI
PCO_SetCmosLineExposure
Delay
dwExposureLines e.g. 5
dwDelayLines
e.g. 4
SC2_SDK_FUNC int WINAPI
PCO_SetInterfaceOutputFor
mat
wDestInterface
SET_INTERFACE_CAME
RALINK (value: 2)
wFormat
SCMOS_FORMAT_TOP
_BOTTOM (value: 0)
3
3
4
1
4
3
2
2
Idle
1
1
2
2
2
3
4
2