®
Polarimeter
PS-2235
4
013-14434A
Insert the Sample Cell
Open the lid of the Polarimeter and place the Sample Cell in the
chamber. The Sample Cell is an exact fit for the length of the
space in the chamber. The direction of the Sample Cell in the
chamber does not make a difference. Close the lid.
Calibration
1.
Pour distilled water into the 100 mm (1 dm) Sample Cell and
insert it in the Polarimeter’s chamber. Close the lid.
2.
Start data collection and slowly rotate the analyzer with the
thumb-wheel so you have data to cover 0° to 360°. For better
analysis, you may want to take multiple passes in this data
range.
3.
Stop data collection.
4.
Record the first angle above 0° where the light intensity is at
a maximum.
To improve the accuracy of this selection, you may wish to apply
a Gaussian fit.
The recorded angle indicates the position where the polarizer and
analyzer are aligned without an optically active substance in the
Sample Cell.
Data Collection
1.
Pour your sample into the 100 mm (1 dm) Sample Cell and
put the Sample Cell in the Polarimeter’s chamber. Close the
lid.
2.
Start data collection and slowly rotate the analyzer with the
thumb-wheel so you have data to cover 0° to 360°. For better
analysis, you may want to take multiple passes in this data
range.
3.
Stop data collection.
4.
Record the first angle above 0° where the light intensity is at
a maximum.
To improve the accuracy of this selection, you may wish to apply
a Gaussian fit.
The recorded angle indicates the position where the polarizer and
analyzer are aligned with an optically active substance in the
sample cell.
The difference between the recorded angles represent the
observed angle of rotation for your sample
Experiment Suggestions
Explore the optical activity of inorganic, organic and biochemical
compounds.
•
Chiral compound (inorganic and organic) purity by optical
rotation.
•
Solution concentration by optical rotation.
•
Determine enantiomeric excess in a mixture of dextro- and
levo-rotatory compounds.
•
Kinetics studies where the reactants or products are optically
active.
For more resources go to
www.pasco.com/polarimeter
Battery
Replacement
If the Polarimeter fails to recharge, the battery may need to be
replaced. Contact PASCO Technical Support for more
information about battery replacement. It is recommended that
the unit be returned to PASCO for battery replacement, if needed.
Troubleshooting the Polarimeter
•
If the Polarimeter loses Bluetooth connection and will not
reconnect, try cycling the ON/OFF button. Press and
briefly
hold the button until the status LEDs blink in sequence, and
then release the ON/OFF button to turn on the Polarimeter.
•
If the Polarimeter stops communicating with the computer
software or tablet application, try restarting the software or
application. If the problem remains, try cycling the ON/OFF
button.
•
As a last resort, push a pin into the Reset port on the bottom
of the Polarimeter.
Technical Support
For assistance with any PASCO product, contact PASCO at:
For more information about the product and the latest revision of
this Reference Guide, visit the PASCO web site at
www.pasco.com and enter “Polarimeter” or the product number
(PS-2235) in the Search window.
Limited Warranty
The Polarimeter has a five year warranty. For a more thorough description
of the product warranty, see the PASCO catalog.
Address:
PASCO scientific
10101 Foothills Blvd.
Roseville, CA 95747-7100
Phone:
+1 916 462 8384 (worldwide)
877-373-0300 (U.S.)
Web:
www.pasco.com
Email:
