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Appendix 

 

Rev. 007 Date 2010-01-13

 

   

CyFlow

®

 space Instrument Operating Manual 

22/26

 

Incubate  for  at  least  30  minutes.  Re-start  the 
system  by  pressing  START  and  let  it  run  to  the 
end. The  Decontamination  Solution  may  incubate 
even  over  night!  Wash  the  flow  cuvette  by 
attaching  a  tube  with  1.6  ml  of  Partec  Sheath 
Fluid and run it for at least 2 minutes. 
 

 
In  case  your  flow  cytometer  shows  degraded 
performance even after thorough cleaning, please 
contact your local distributor or Partec. 
 
 
 

 

Maintenance and Service  

 

 
Maintenance 

Clean the CyFlow

®

 space casing on a regular base, carefully with 

soft cloth. Water must not enter the CyFlow

®

 space or peripheral 

devices or come into contact with electric connections and 
switches. For cleaning the screen, always use special screen 
cleaner and soft cloth. 

 

 

 

Do not use

 any organic solvents, nitro thinner, benzol, alcohol, 

highly concentrated bleach etc! 

 
 

For cleaning of flow cuvette, refer to page 21. Do not use tools to 
clean the flow cuvette. In case the flow cuvette is blocked, 
enquire Partec for rapid exchange. 

  
 

Regularly empty the waste bottle and clean with warm detergent 
solution and a brush. 

 

 

 

Clean sheath reservoir with destilled water and a clean brush and 
flush with clean destilled water several times. Remember 
cleanliness of sheath fluid reservoir is critical for proper 
operation. 

 
 

If the CyFlow

®

 space will not be used for longer periods, clean 

flow system by using distilled water. Put a sample tube half-ways 
filled with destilled water at the sample port. Clean waste and 
sheath reservoir, wipe top dry. 

 
Service 

All service is to be made from an authorized service engineer. 
Please contact your supplier or partec for service requests. 

 

 

 

Transport and Storage 

For  the  transport  of  the  system  to  a  different 
location  it  will  be  necessary  to  disconnect  all 
external  tubing,  data,  and  supply  connections.  In 
case  of  use  with  potentially  biohazardous 
material,  please  see  Partec  standard  operating 
procedure  (SOP)  for  decontamination.  The 
system  should  be  carried  in  upright  position. 
During  transport  or  storage  please  take  care  that 
the  system  will  be  stored  under  the  following 
conditions: 
 
Temperature 

5-50 C 

Humidity 

20-85% relative (non-condensing) 

Room 

Clean  environment,  no  direct  sun 
light 

 

Disposal  

 
In  case  of  product  disposal,  please  proceed 
according  to  the  Partec  standard  operating 
procedure (SOP) for decontamination. 
After  decontamination,  the  system  has  to  be 
disposed  according  to  the  local  regulations  and 
laws.  
For  further  information,  please  contact  your  local 
distributor or Partec. 

Содержание CyFlow space

Страница 1: ...CyFlow space Instrument Operating Manual...

Страница 2: ...ent Settings 12 The Parameter Setup Dialog Box Pulse Height Area and Width 13 Trigger 14 PMT High Voltage Gain and Log Amplification 15 Sample Speed 16 Threshold Lower Level L L 17 Appendix 18 Install...

Страница 3: ...lly any flow cytometric application The applications cover e g Routine and research Immunophenotyping Blood Cell Analysis HIV monitoring Leukocyte Counting Rare Event Analysis Microorganism Analysis F...

Страница 4: ...haracteristic colour emission wavelength spectrum This fluorescence light is separated into colour ranges by means of optical filters The intensity of each colour range is analysed for each single cel...

Страница 5: ...ometer is operated with 100 240 V AC Switch on main power at the left side of the instrument The 488nm laser has its own power button closeby please also switch on the 488 nm laser All other lasers ar...

Страница 6: ...s of the flow cuvette The CyFlow space Flow Cytometer supports either 2 laser spots when employing FloMax version 2 6 or 2 7 or 3 laser spots when employing FloMax version 3 0 The blue 488 nm laser wh...

Страница 7: ...nsert sample tube onto the sample port until you recognize a click The sample should be fully mounted within a second Now the measurement acquisition starts automatically the operating software indica...

Страница 8: ...ath fluid tube for 5 seconds Stop the instrument while keeping the tube pinched Incubate for 15 minutes Re start the system by pressing START and let it run to the end Run the system with 1 6 ml Parte...

Страница 9: ...ential This requires fast recognition and analysis of the events by electronics and computer All Partec instruments are specifically designed to minimize counting losses by providing direct connection...

Страница 10: ...No additional analysis steps e g setting gates for beads are required v Less Expenses No reference beads required Performing True Volumetric Absolute Counting Sedimentation and Count Time Cells or oth...

Страница 11: ...rward scatter SSC side scatter FL1 green fluorescence FITC FL2 orange fluorescence PE FL3 red fluorescence I PE Cy5 FL4 far red fluorescence I PE Cy7 FL5 red fluorescence II APC FL6 far red fluorescen...

Страница 12: ...ion for the particles of interest The adjustments cover the gains of the optical detectors e g the photomultiplier high voltages the amplification mode lin 3 or 4 decade logarithmic lower and upper le...

Страница 13: ...meter label To change parameter names click into the fields and enter the names by using the keyboard For each optical channel you may analyse three different pulse properties 1 The pulse height 2 the...

Страница 14: ...l other particles will not be recognized by the instrument The trigger parameter can be used for an efficient exclusion of unwanted particles from the analysis Example Assume vertebrate leukocytes in...

Страница 15: ...the instrument settings box see Fig 4 By using the Right Left buttons gain values are increased decreased In case linear amplification is used peaks are expanded to the right compressed to the left w...

Страница 16: ...decreased by clicking into the corresponding field in the instrument settings box and using the Right Left buttons see Fig 4 The count rate increases with elevated speed values If the speed is increas...

Страница 17: ...of small and unwanted background or noise signals below a threshold The L L value of a parameter can be increased decreased by clicking into the corresponding L L value field in the instrument setting...

Страница 18: ...educe smoke dust vibrations direct sunlight and direct neighbourhood of heatings as possible The installation room must be well ventilated and dry Temperature 15 30 C Humidity 20 85 relative non conde...

Страница 19: ...space shown from the rearside CyFlow rearside Screen Computer rearside Printer CCD Camera Interface Monitor Printer CCD Computer AC AC AC Air Sheath Mouse Keyboard Sheath Waste AC Waste CyFlow rearsid...

Страница 20: ...e supply sheath pressure and sample flow rate The Computer connections of the CyFlow space are located at the rear side of the instrument Connect the CyFlow space to the computer with the interface ca...

Страница 21: ...during sample run remove your sample and activate CLEAN several times In this way the flow cuvette is back flushed with sheath fluid Restart the sample and observe peak resolution again If the results...

Страница 22: ...lled water and a clean brush and flush with clean destilled water several times Remember cleanliness of sheath fluid reservoir is critical for proper operation If the CyFlow space will not be used for...

Страница 23: ...1 2001 Warning Laser light can be emitted if the protection cover for the laser beam is removed and the beam shutter is opened Therefore the system is marked with the following laser safety labels War...

Страница 24: ...entation control Particle Concentration Analysis True Volumetric Absolute Counting Particle Size and Fluorescence Distribution Analysis True Volumetric Based on precise counting and mechanical fluid v...

Страница 25: ...d precision syringe pump for contamination free sample transport Built in air pressure for sheath fluid Sheath fluid pressure is adjustable from 0 300 mbar Computer controlled Default setting 200 mBar...

Страница 26: ...d to 32 gates in free logical combinations Regions quadrants 1P ranges Gating Crosstalk On or offline gating and crosstalk compensation provide adjustments without need Compensation to rerun samples D...

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