Oxford Benchmate 54P-D, Руководство пользователя, Страница: 12 / 16

10. TIPS FOR EFFICIENT OPERATION
Orbital shaking is a simple way for the mixing of assay components, but
simply putting the samples in a micro-plate does not guarantee reaching
complete blending after the mixing process.
The adjustment of the optimal mixing speed for micro-plates is dependent
on the size of the well and the filling volume. The below table is a short
summary for recommended mixing speeds for different micro-plates.
Mixing Speeds (RPM) for Different Micro-plates Depending on Filling
Volume/Well(%)
Filling Volume in % 96 Standard Well 96 Deep Well 384 Standard Well
50% 1000 -1200 RPM 800 -1200 RPM 1200 RPM
75% 800 -1000 RPM 600 -1000 RPM 900-1100 RPM
Larger volumes should be mixed with a higher orbit and low speed. Smaller
volumes requires a lower orbit, but a much higher speed.
Note: Overfilling of wells can lead to spillage.
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