Mosaic3
47
Version 3.5 rev 8 May 2019
OPERATION
5.4.7 o
verLap
m
ode
This applies to Sequencing operational mode and will remove any gaps between exposures so there will continually be a
mask displayed and a minimum possible gap during mask transition. The DMD Active output signal will give a short (~5
µs) dip to allow mask counting or the synchronisation of other hardware.
5.4.8 e
xternaL
t
riggering
An external digital trigger signal can be used to trigger an individual exposure in either Single Shot Mode or Referencing
Mask Memory Mode. When used with Sequencing Mask Memory Mode the External Trigger signal will trigger the
exposure of the currently loaded mask, and then increment and load the next mask in the sequence.
The signal can be interfaced via either the Ext. Trigger SMA connector or Pin 1 of the D-Type External Interface
connector. In all Operational modes there is a 200 µs delay between the rising edge of the trigger signal and the change
in optical output.
5.4.9 e
xternaL
f
rame
a
ddressing
Pins 17-10 of the D-Type External Interface connector accept digital signals to specify a mask memory address from 0
-138 as a binary address [7:0].
5.4.10 d
evice
s
ynchronization
(dmd a
ctive
o
utput
)
The DMD Active Output produces an Active High digital signal when the DMD is exposing a mask. This can be used to
hardware synchronise an additional piece of equipment (e.g. light source for DMD illumination). The signal goes high 4
µs before the Optical Output changes and ends 3 µs after the DMD finishes exposing.
5.4.11 b
eam
s
pLitter
p
Lug
o
peration
The beam splitters are each contained in an interchangeable, kinematic type mount that we call a Beam Splitter Plug. A
Beam Splitter Plug can contain optical elements to:
•
Completely block transmission of light from the primary light source while allowing all the light from the
secondary source to pass into the microscope.
•
Provide both primary and secondary light to enter the microscope using a dichroic or other type of mirror.
•
Allow only light from the primary source to pass through to the microscope, i.e. no optical element.
When installed, Beam Splitter Plugs are held in place in their respective beam splitter housings by magnets and
kinematic mounts that assure repeatable alignment.
Warning: Before changing a Beam Splitter Plug verify that the laser is turned off. Do not insert any object or
body part into the Beam Splitter Plug aperture, even if the system is not engaged or powered on.
Removal of a beam splitter plug is performed by grasping the large grey knob on the top of the plug, giving the knob a
slight twist (this helps break the magnetic field) and lifting the assembly from the housing or block, as the case may be.
Replacement of a beam splitter plug is performed by placing the selected plug into the block or housing desired and
rotating the plug after it contacts the bottom until a strong (magnetic) detent is felt.
Note: Removing the beam splitter plug will make the system inoperable. A safety interlock ceases all
emission from the laser until the beam splitter is properly reinstalled.
Warning: Ensure that the Beam Splitter Plug is correctly orientated before the system is enabled.
