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Use a piece of lint-free paper or an eyedropper to place a small droplet of oil directly on the
front lens of the immersion objective. Next, place another droplet of oil on the cover glass
immediately above the area to be imaged.
•
Next, quickly rotate the oiled objective into position above the specimen merging the two oil
droplets (one on the sample and one on the objective) into a single pool. The specimen is
now ready for observation and photomicrography.
•
As you scan the surface of an oiled microscope slide, periodically observe the position of
the stage with respect to the objective and condenser front lens to assure that there is a
continuous bead of oil between these elements and the microscope slide. When scanning
over a large area on a microscope slide, it is often necessary to reapply some oil to assure
continuity of the imaging medium.
Keep The Lenses Clean and Oil-Free
Always strive to make sure that your microscope objective and substage condenser front
lenses are kept clean and free of immersion oil. Use a Q-tip or lint-free paper or cloth to
gently wipe excess oil from the lenses and microscope slide after experiments are finished.
After the oil is removed, use a suitable solvent to remove traces of immersion oil from the
lenses. Failure to clean lenses properly may result in small crystallites forming on the coa
-
ted surfaces when immersion oils either dry or collect dust and other contaminating particles
from the atmosphere.
Mains Power:
90-240 V, 50/60 Hz
Light source: 3W high-efficiency white LED
5.0 TECHNICAL TIPS FOR OIL IMMERSION MICROSCOPY
6.0 ELECTRICS
