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17. Let sit at room temperature for 2-3 minutes.

18. Place the plate on a magnetic separation device to magnetize the Mag-Bind®

Total Pure NGS. Let sit at room temperature until the Mag-Bind® Total Pure NGS is
completely cleared from solution.

19. Transfer the cleared supernatant containing purified DNA to a new 384-well

microplate and seal with non-permeable sealing film.

20. Store the plate at 2-8°C if storage is only for a few days. For long-term storage,

samples should be kept at -20°C.

Mag-Bind® Total Pure NGS - 384-well Plate Protocol

Содержание Mag-Bind M1378-00

Страница 1: ...Mag Bind Total Pure NGS M1378 00 5 mL M1378 01 50 mL M1378 02 500 mL August 2016...

Страница 2: ...ions 4 Storage and Stability 4 Mag Bind TotalPureNGS96 wellPlateProtocol 5 Mag Bind TotalPureNGS 384 wellPlateProtocol 8 Troubleshooting Guide 11 Ordering Information 12 Manual Revision August 2016 In...

Страница 3: ...solution for nucleic acid purification compared to centrifugation and vacuum based technologies The product can be easily scaled up while providing very user friendly handling procedures If using Mag...

Страница 4: ...3 Illustrated Protocol Determine the Reaction size Add Mag Bind Total Pure NGS and Mix Magnetize and Remove Supernatant Wash Twice with 70 Ethanol Dry Elute DNA...

Страница 5: ...e and Stability Mag Bind Total Pure NGS is guaranteed for at least 12 months from the date of purchase when stored at 2 8 C Kit Contents Product Number M1378 00 M1378 01 M1378 02 Mag Bind Total Pure N...

Страница 6: ...d 2 Place the 96 well PCR plate on the bench and measure the volume of the PCR reaction Determine the volume of Mag Bind Total Pure NGS that will be added to the reaction If the reaction volume will e...

Страница 7: ...d the Mag Bind Total Pure NGS 11 Aspirate and discard the cleared supernatant Do not disturb the Mag Bind Total Pure NGS 12 Repeat Steps 9 11 for a second 70 ethanol wash step 13 Leave the plate on th...

Страница 8: ...re until the Mag Bind Total Pure NGS is completely cleared from solution 19 Transfer the cleared supernatant containing purified DNA to a new 96 well microplate and seal with non permeable sealing fil...

Страница 9: ...etic separation device if provided 2 Place the 384 well PCR plate on the bench and measure the volume of the PCR reaction Transfer the sample to a skirted 384 well PCR plate 3 Shake the Mag Bind Total...

Страница 10: ...al Pure NGS 11 Aspirate and discard the cleared supernatant Do not disturb the Mag Bind Total Pure NGS 12 Repeat Steps 9 11 for a second 70 ethanol wash step 13 Leave the plate on the magnetic separat...

Страница 11: ...ure until the Mag Bind Total Pure NGS is completely cleared from solution 19 Transfer the cleared supernatant containing purified DNA to a new 384 well microplate and seal with non permeable sealing f...

Страница 12: ...me Aspirate slowly DNA remains bound to beads Increase elution volume Incomplete resuspension of the beads during elution Vortex or pipet up and down to fully resuspend the beads Problem Cause Solutio...

Страница 13: ...832 8896 Product Part Number Mag Bind Total Pure NGS 50 mL M1378 01 Mag Bind Total Pure NGS 500 mL M1378 02 Elution Buffer 100 mL PDR048 HiBind E Z N A Mag Bind and MicroElute are registered trademark...