Optical microscope accessory
3
Operation
Adjusting the microscope
Turn ON the light source of the microscope and adjust required portions
of the microscope to make it ready for observation.
Set the light path of the trinocular tube to the camera light path.
Adjusting the camera and monitor
Adjust colors, etc. according to the instruction manual of the camera
and monitor in use.
(The center of the field of view through the eyepieces and that of the
image acquired by the camera may not coincide completely, but this
is a matter of function of the image sensor adjustment mechanism of
the camera, and not a malfunction.
Adjusting the parfocality between the visually observed image
and the image on monitor.
By adjusting the parfocality, the image won’t be defocused when
the observation light path of the microscope is changed.
Before adjusting the parfocality, adjust the diopter of the microscope
in advance. For procedures to adjust the diopter, refer to the
instruction manual for the microscope in use.
Use the Allen screwdriver provided with the microscope for adjusting
the parfocality.
The parfocality adjustment range is ±0.8 mm. If the
parfocality cannot be adjusted completely with the
adapter, use the focusing function of the camera as well.
Change the objective of the microscope to the high magnification
objective, look into the eyepieces and bring the specimen into focus.
Change the objective to the low magnification objective and check
that the image is in focus by looking through eyepieces. Then, change
to the camera light path and observe the image on monitor.
Loosen the clamping screw (LOCK) A of the C mount camera adapter
using the Allen screwdriver.
While observing the image on monitor, adjust its focus by rotating
the parfocality adjustment screw (FOCUS) B slowly using the Allen
screwdriver.
When it is in focus, tighten the clamping screw (LOCK) A using the
Allen screwdriver.
Adjusting the direction between the visually observed image and the image on monitor.
Loosen the clamping screw C of the camera port of the trinocular tube.
Rotate the C-mount camera adapter so that the direction of the visually
observed image coincides with that of the image on monitor, and
tighten the clamping screw C firmly.
1
2
NOTE
1
2
3
4
5
1
2
4
Imaging field area
Magnification on monitor = Objective magnification x C mount camera adapter magnification (0.63X) x Monitor diagonal length
Image sensor diagonal length
*The diagonal length of the monitor and that of the image sensor differ depending on manufacturers.
Image sensor
Reference data
1 inch: 16.16 mm, 1/1.2 inch: 13.4 mm, 2/3 inch: 11 mm, 1/2 inch: 8.08 mm, 1/3 inch: 6 mm, 1/4 inch: 4 mm
B
A
C
For your reference, the following diagram shows the areas of
imaging field determined by the field of view when eyepieces
with a field number of 22 or 26.5 are used and the size of the
image sensor through the C-mount camera adapter.
TIP
2/3 inch image sensor
1/2 inch image sensor
1inch image sensor
Field
number
26.5
Field
number
22
