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  OPTICAL CHARACTERISTICS

Optical character

UIS2 objective

Magnification

N.A.

W.D.

(mm)

Cover glass 

thickness

(mm)

Resolution

(μm)

Eyepiece WHN10X (FN22)

Remark

Total mag.

Depth  

of focus 

(μm)

Field of 

view

PLN-P
Plan Achromat for 
polarized light (FN22)

4X

0.10

18.5

– 

3.36

40X

180.0

5.5

ACHN-P
Achromat for polarized 
light (FN22)

10X

20X
40X

100XO

0.25
0.40
0.65

1.25

6.0
3.0

0.45

0.13

0.17
0.17
0.17

1.34

0.84
0.52
0.27

100X

200X
400X

1000X

28.0

  6.09
  3.04
  0.69

2.2

1.1

 0.55
 0.22

Oil immersion

UPLFLN-P
Plan Semi Apochro-
mat for polarized light 
(FN26.5)

4X

10X

20X
40X

100XO

0.13

0.3
0.5

0.75

1.3

17.0

10.0

2.1

0.51

0.20


0.17
0.17
0.17

2.58

1.12

0.67
0.45
0.26

40X

100X

200X
400X

1000X

83.6

14.7

  4.60

  1.66

  0.43

5.5
2.2

1.1

 0.55
 0.22

Oil immersion

– UIS series objectives not listed here can also be combined with this microscope. –

The table below shows the optical characteristics of different 
eyepiece and objective combinations. Objective specifica-
tions are marked on the objective (as shown in the diagram 
on the right).

 
Refer to the latest catalogue or consult your local Olympus 
representative for the updated information on the eyepieces 
and objectives that can be combined with this microscope.

NOTE

Magnification

Mechanical 

tube length

Designation

Color band

Numerical aperture (N.A.)

Field number (FN)

Cover glass thickness

Содержание BX53M-P

Страница 1: ...with the use of your microscope we recommend that you read this manual together with the instruction manual for the BX53 BX53M microscope thoroughly before use Retain this instruction manual in an ea...

Страница 2: ......

Страница 3: ...4 2 Intermediate Polarizing Attachment 15 5 POLARIZED LIGHT OBSERVATION 16 5 1 Adjustments Before Observation 16 1 Adjusting the Optical Axis 16 2 Adjusting for Extinction 22 3 Adjusting the Eyepiece...

Страница 4: ...TION 1 Getting Ready 1 A microscope is a precision instrument Handle it with care and avoid subjecting it to sudden or severe impact 2 The BX53 BX53M microscope can be used with up to two additional i...

Страница 5: ...ight observation Strain free objective series Strain free condenser BX53 BX53M Microscope frame Observation tube Centerable revolving nosepiece Polarizing intermediate attachment Eyepiece 1 1 NOMENCLA...

Страница 6: ...ng any parts or touching glass surfaces CAUTION Light source unit Eyepiece Centerable revolving nosepiece U P4RE Test plate adapter U TAD Stage clips Mechanical stage U FMP Polarizing rotatable stage...

Страница 7: ...age bracket with the positioning pin on the stage aligned with the groove on the front of the stage bracket then loosely tighten the centering knobs Mounting Stage Clips and Mechanical Stage U FMP Fig...

Страница 8: ...limit 3 Loosen the condenser clamping screw 4 Position the condenser with the scale markings in front insert it into the substage fork as far as it will go Align the positioning pin at the back of th...

Страница 9: ...reases from low to high power in a clock wise direction from the primary hole Fig 4 Low Fig 5 High 4 Mounting the Revolving Nosepiece U P4RE Fig 5 1 Turn the coarse adjustment knob to lower the stage...

Страница 10: ...ening on the microscope frame and clamp by tightening the clamping screw When Using Intermediate Attachment for conoscopic and orthoscopic observation U CPA Always make sure to mount this unit in such...

Страница 11: ...intermediate attachment placing the observation tube to point the binocular eyepieces towards the front Fasten the observation tube by tightening the clamping screw Insert the eyepiece with cross line...

Страница 12: ...AN360P place the fixed analyzer in the test plate adapter U TAD Position the fixed analyzer so that the positioning pin on the fixed analyzer fits into the groove The fixed analyzer will be held in p...

Страница 13: ...nts are introduced on the following pages Bertrand lens knob Slider clamping knob Mechanical stage Aperture iris diaphragm lever Polarizer rotation ring Bertrand lens focusing knob Analyzer Slot for c...

Страница 14: ...60P Test Plate Adapter U TAD Sensitive Tint Plate U TP530 Quarter Wave Plate U TP137 Analyzer slot Stopper knob Clamping knob Rotation dial Hole for the fixed analyzer U ANT Test plate clamping knob S...

Страница 15: ...e clips When Using the Mechanical Stage U FMP Fig 12 Open the spring loaded finger and place the specimen on the stage Applicable slide glass is for inorganic substances 28 x 48 mm and organic substan...

Страница 16: ...top lever located at the right side of the stage is moved toward the observer and the stage is moved from this position to the first click stop position the specimen is moved 45 to its diagonal positi...

Страница 17: ...the stage from the microscope See page 4 2 Using the Allen screwdriver loosen the substage bracket clamping screw and remove the substage assembly Fig 15 3 Turn the coarse adjustment knob and raise th...

Страница 18: ...ediate Polarizing Attachment 2 Using the Analyzer Fig 18 3 Focusing the Conoscopic Image U CPA only Fig 18 The analyzer is engaged when pushed in to the second click stop position To disengaged the an...

Страница 19: ...ig 19 2 Remove the condenser 3 Insert the rotatable analyzer U AN360P into the analyzer slot on the intermediate attachment Engage the analyzer loosen the clamping screw and set the analyzer s rotatio...

Страница 20: ...Place the specimen 2 Focus on the specimen and look for an easily recognizable detail in the field Move this detail in the center of the eyepiece cross lines 3 When the stage is rotated the detail mov...

Страница 21: ...ght path one after another When Using Intermediate Attachment for conoscopic and orthoscopic observation U CPA Normal Observation Make sure the 10X objective is inserted into the primary hole on the c...

Страница 22: ...ing Intermediate Attachment for conoscopic and orthoscopic observation U CPA Photomicrography 1 Using the tip of a mechanical pencial etc push out in the direction of the objective hole the black rubb...

Страница 23: ...mage Fig 27 7 Insert the two provided centering wrenches into the centering holes for the primary hole with the 20X or 10X objective on the nosepiece 8 Manipulate the centering wrenches to bring the b...

Страница 24: ...will move the specimen detail in the opposite direction away from the cross line center 4 Moving the specimen only move a new specimen detail into the cross line center Repeat steps 3 and 4 several t...

Страница 25: ...10X objective 2 Insert the rotatable analyzer into the light path and set vibration direction scale at the 0 position Clamp with the clamping knob Fig 30 3 Set the polarizer scale at the 0 position 4...

Страница 26: ...provided with the U CPA or U OPA is required Orientation plate U PJ Single module name Remove the test plate adapter and the compensator from the light path 1 Swing out the top lens of the condenser 2...

Страница 27: ...ving rotate the stage to locate the position where the orientation plate is darkest At this point clamp the stage 5 Remove the analyzer from the light path Set to brightfield and slightly loosen the o...

Страница 28: ...the Bertrand lens from the light path 2 Insert the analyzer and perform observation When the top lens is swung out the aperture iris diaphragm and field iris diaphragm will not function nor mally If t...

Страница 29: ...he aperture iris diaphragm 6 Slide the focusing knob on the U CPA to focus on the conoscopic image When the intermediate attachment conoscopic and orthoscopic observation U CPA is not used conoscopic...

Страница 30: ...e analyzer U AN360P 2 Analyzer U AN360P 360 dial rotation Minimum scale reading 0 1 Vernier scale 3 Revolving nosepiece U P4RE Type Centerable quadruple Attachable compensators Quarter wave plate U TP...

Страница 31: ...lens swung in Applicable objectives 4X to 100X 4X objective is used with the top lens swung out Operating environment Indoor use Altitude up to 2000 m Temperature 5 to 40 C 41 to 104 F Maximum relati...

Страница 32: ...100XO 0 13 0 3 0 5 0 75 1 3 17 0 10 0 2 1 0 51 0 20 0 17 0 17 0 17 2 58 1 12 0 67 0 45 0 26 40X 100X 200X 400X 1000X 83 6 14 7 4 60 1 66 0 43 5 5 2 2 1 1 0 55 0 22 Oil immersion UIS series objectives...

Страница 33: ...e light path 15 b Vignetting or uneven field of view Test plate is stopped at an intermediate position Set at click stop position 9 During orthoscopic observation the condenser top lens is placed in t...

Страница 34: ...MEMO...

Страница 35: ......

Страница 36: ...ter Our Website https www olympus ims com service and support service centers https www olympus ims com This product is manufactured by EVIDENT CORPORATION effective as of Apr 1 2022 Please contact ou...

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