Olympus BX-FLA Скачать руководство пользователя страница 44 | Manualshive

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Содержание BX-FLA

Страница 1: ...ety and obtain optimum performance and to familiarize yourself fully with the use of this attachment we recommend that you study this manual thoroughly before operating the microscope system Retain th...

Страница 2: ...rds are properly connected 4 The ultraviolet rays emitted by the burner are harmful Be sure to use a UV protective shield with the unit See page 9 5 Do not open the lamp housing while the burner is tu...

Страница 3: ...n adjuster changer rsal reflected light fluorescence vertical illuminator U URA Conoscope attachment w Microscope frame BX40 or BX50 l U OPA Orthoscope attachment Multi viewing attachment U DA Drawing...

Страница 4: ...BX FLA...

Страница 5: ...ttance and reflect ance it is necessary to use an appropirate combination of exciter and barner filters in conjunction with the dichroic mirror This is necessary to achieve a good contrast image throu...

Страница 6: ...Universal reflected light fluorescence vertical illuminator I IMercury lamp housing Power supply unit BX series microscope I I I Universal condenser 0 2...

Страница 7: ...Light excluding shutter Filter slider Another is on opposite side Slider insertion slot 1 B Cube Removable index mark Combine with dichroic mirrors barrier filters and exciter filters as appropriate f...

Страница 8: ...C Fluorescent Light Source l Mercury Burner Lamp Housing Burner centering knob Burner Socket l Power Supply Unit Burner ON LED Main switch AC receptacle output connector 0 4...

Страница 9: ...assemble the parts in the sequence indicated by the numbers in the illustration below Eyepiece Observation tube nrversal reflected orescence vertic UV protective shield Light excluding slide Microsco...

Страница 10: ...ponding turret position Fig 2 Ir Use a sharp object such as the tip of a ballpoint pen or mechanical pencil to lift the cube s magnetic index sticker Ir Move the cube s magnetic index sticker WU NU et...

Страница 11: ...sing are removed Mounting the Collector Lens 1 Align the collector lens positioning groove with the pin inside the lamp housing pull up the collector lens focusing knob 0 and slide the collector lens...

Страница 12: ...urner when it has been used for 200 0 hours The burner may crack if used beyond the specified life time 2 Before replacing the burner wait at least 10 minutes after turning the burner off Before remov...

Страница 13: ...to the AC receptacle on the power supply unit then plug the other end of the cord into an AC outlet A Connect the power cord correctly and ensure that the ground terminal of the power supply and that...

Страница 14: ...s l Barrier filter 0 1 25 O mm dia 6 mm max thickness l Exciter filter 0 1 0 1 l Dichroic mirrors 26 0 3mm X 38 0 3mm 1 0 05 mm thickness Barrier filter commercially available Dichroic mirror commerci...

Страница 15: ...econds Ir To avoid shortening the life of the burner do not turn the burner off within 15 minutes of ignition After turning the burner off it cannot be re ignited until the mercury vapor cools and con...

Страница 16: ...ight path 5 Pull out the aperture iris diaphragm knob to bring the shadow of the aperture iris diaphragm into the centering screen BH2 SGRF 6 Adjust the two aperture iris diaphragm centering knobs to...

Страница 17: ...of revolving nosepiece or objective lens and engage the empty hole in the light path 4 Pull out the field iris diaphragm knob to close this iris and push in the aperture iris diaphragm knob to fully o...

Страница 18: ...h If neither of these cubes is available move some other fluorescent cube into the light path 3 Screw the centering screen BH2 SGRF into the revolving nosepiece and move it into the light path 4 Push...

Страница 19: ...filters with the marked side facing toward the observer As you insert a filter you will hear two clicks At the first the filter is at the empty position and at the second it enters the light path Not...

Страница 20: ...505 BP450480 BP470490 BP420480 BP460 490 BP470490 BA515 BA515lF l FITC l Acridine orange DNA RNA l Auramine Band Pass Barrier Filter Combinations I I U U MNUA DM400 BP360 370 BA420 460 For observing o...

Страница 21: ...Apo 20x 40x 40x01 ReSlecteti u v BV 0 0 0 0 0 0 0 0 0 0 0 0 Transtiittred Transmitted phase Nomarski contrast DIG 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Recommended combination Ox Usable but image may be dark...

Страница 22: ...vation transmitted light phase E contrast observation and transmitted light differential interference contrast observation as well as reflected 2 light fluorescence observation With specimens th at fa...

Страница 23: ...IC slider to adjust contrast of the differential interference m contrast image l 9 Move the cube corresponding to the desired excitation into the light path and open the light excluding shutter 2 10 A...

Страница 24: ...00 a Wavelength nm Wavelength nm 2 U excitation cube Narrow band 5 BV excitation cube Narrow band 100 __ ___ ___ DM400 BA420 _______ DM455 1 I 40d I I I 600 I Wavelength nm 1 Waveilength nm 3 V excita...

Страница 25: ...I I 600 I 600 800 3OC Wavelength nm Wavelength nm 8 B excitation cube Surer wide band II G excitation cube Wide band p iiiq p i q I I I I 7 I I I 400 600 800 Wavelength nm 100 I 8 I I 7 I 7 BA590 BP5...

Страница 26: ...h nm Wavelength nm 14 IG excitation cube Wide band 17 IB excitation cube for color separation Wide band U MWlBAl 1001 s I I 8 I 8 I L 600 800 Wavelength nm Wavelength nm 15 IY excitation cube Wide ban...

Страница 27: ...ercury Burner Spectrum 3651366 100 r s 312 6 313 2 300 400 500 600 700 800 Wavelength nm For fluorochrome emission a light beam having a specific wavelength is selected from a wide spectrum of wavelen...

Страница 28: ...become possible by combining the universal reflected light vertical illuminator U URA with metallurgical UIS objectives brightfield cube U MBF or darkfield cube U MDF Power a lamp housing Objective BX...

Страница 29: ...cal Illuminator shown on page 3 1 B Cube For reflected light observation with white light use a brightfield cube U MBF a darkfield cube U MDF and a differential interference contrast tube U MDIC etc 1...

Страница 30: ...enon burner for darkfield observation illumination at the periphery may be slightly insufficient depending on the specimen If the brightfield cube U MBF is engaged while the mercury burner or xenon bu...

Страница 31: ...etic index sticker I and affix it to the turret Fig 2 Use a sharp object such as the tip of a ballpoint pen or mechanical pencil to lift the cube s magnetic index sticker Move the magnetic cube index...

Страница 32: ...e the bulb clamping levers to their original positions to secure the bulb Figs 6 7 Do not touch the bulb with bare hands If fingerprints or other smears are accidentally left on the bulb wipe it clean...

Страница 33: ...place by the magnet on the underside of the vertical illuminator 3 Reattach the revolving nosepiece I Connect the lamp housing cord connector to the lamp housing connector I on the power supply unit 2...

Страница 34: ...the revolving nosepiece to engage the 1OX objective then place a specimen on the stage and bring the image into approximate focus 2 Pull out the field iris diaphragm knob iJ on the universal fluoresc...

Страница 35: ...rture of the illumination system to control the depth of focus contrast resolution etc 0 Reflected light darkfield observation Always keep the aperture iris diaphragm knob pushed in to leave the diaph...

Страница 36: ...eld observation or if needed to shorten the exposure time during photomicrography or to brighten the field of view during darkfield observation remove the filter from the light path When using the DIC...

Страница 37: ...select the interference color of the background and to achieve the maximum contrast depending on the specimen under observation as outlined below Fig 18 2 Rotating the prism control knob of the U DICR...

Страница 38: ...erference Contrast Observation outlined on page 32 1 Place the specimen on the stage and move the stage to bring the specimen into focus Simple polarized light observation is now possible 2 Adjust the...

Страница 39: ...0 80 22 5 21 0 12 0 10 6 3 4 2 58 1 34 0 84 0 67 0 42 50x 100x 200x 500x 1000x 70 1 18 4 6 09 2 50 0 87 4 4 2 2 1 1 0 44 0 22 75x 150x 300x 750x 1500x 75x 150x 300x 750x 1500x 52 2 13 7 4 64 2 03 0 72...

Страница 40: ...the image circumscribes the field of view A cube unsuitable for the specimen is used Change to suitable cube The objectives are not inserted into the light Rotate the revolving nosepiece until it pat...

Страница 41: ...lens of the objective is dirty Immersion oil is not being used with an oil immersion objective Recommended immersion oil not used DF insert not mounted The specimen is placed in a tilted position on t...

Страница 42: ...ns Lamp Housing Collector lens focusing knob Not used with a halogen bulb Lamp Lamp up down adjustment knob I Lame focusina I PHILIPS 7724 long life type or Lamp left right PHILIPS 7023 high brightnes...

Страница 43: ...off the main switch and wait for bulb lamp socket and lamp house to cool down Fig 21 Use only the 12V IOOW halogen lamp PHILIPS 7724 or 7023 Do not touch the halogen bulb with bare hands If the bulb...

Страница 44: ...t circuit etc or in case of overcurrent When they activate the center portion of the breaker will pop up and the power will be cut off To reset the breakers press in the center portion If a breaker ac...

Страница 45: ......

Страница 46: ...er Drive Melville NY 11747 3157 U S A OLYMPUS SINGAPORE PTE LTD 491 B River Valley Road 12 01 04 Valley Point Office Tower Singauore 248373 OLYMPUS OPTICAL CO kKJ LTD 2 8 Honduras Street London EClY O...

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