OligoMaker X12 Скачать руководство пользователя страница 3 | Manualshive

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OligoMaker X12 Скачать руководство пользователя страница 3

OligoMaker DNA/RNA Synthesizer

OligoMaker ApS

•

Kong Georgs Vej 12

•

DK-2000 Frederiksberg

•

Tel: +45 321 322 00

www.oligomaker.com

•

email:

[email protected]

p.3

1. Introduction

This manual is a guide in how to operate the OligoMaker X12.

OligoMaker is produced by OligoMaker ApS, Kong Georgs Vej 12,
DK-2000 Frederiksberg, Denmark -

www.oligomaker.com

OligoMaker ApS obtained CE marking in May 2009

This manual must be kept in the laboratory next to the OligoMaker.

The responsible operator or personnel of the OligoMaker must read these instructions
carefully.

2. Symbols and abbreviations

Bottle holders:

T

T amidite solution

A

dA amidite solution

G

dG amidite solution

C

dC amidite solution

1

Extra amidite solution

2

Extra amidite solution

3

Extra amidite solution

4

Extra amidite solution

5

Extra amidite solution

6

Extra amidite solution

7

Extra amidite solution

8

Extra amidite solution

9

Extra amidite solution

0

Extra amidite solution

Act

Activator solution

Sol

Solvent (ex. Acetonitrile)

Deb    Deblock solution
Cap A   Capping A solution
Cap B   Capping B solution
Ox1    Oxidizer (iodine) solution
Ox2    Oxidizer (thio) solution

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Содержание X12

Страница 1: ...DNA RNA Synthesizer OligoMaker ApS Kong Georgs Vej 12 DK 2000 Frederiksberg Tel 45 321 322 00 www oligomaker com email karl ross dk p 1 Manual January 2nd 2019 DNA RNA Synthesizer 2019 OligoMaker X12...

Страница 2: ...4 TERMS 11 5 APPLICATION 11 6 SAFETY 12 7 TRANSPORTATION 13 8 INSTALLATION 13 8 1 A QUICK GUIDE 14 9 SYNTHESIS START A USERS GUIDE 16 9 1 CONTROL OF COLUMNS 16 9 2 CHECK REAGENTS 17 9 3 PRIMING 18 9 4...

Страница 3: ...responsible operator or personnel of the OligoMaker must read these instructions carefully 2 Symbols and abbreviations Bottle holders T T amidite solution A dA amidite solution G dG amidite solution C...

Страница 4: ...l karl ross dk p 4 3 Touch screen manual 3 1 Opening Screen Prime Opens for Priming section 3 3 Manual control Opens for Manual Control section 3 2 Get data Imports data section 3 4 Delete Oligo Opens...

Страница 5: ...ram that fills all the columns with the desired amount of acetonitrile following with activating of Pump Run You can check whether all columns have been filled with solvent and subsequently sucked dry...

Страница 6: ...valve from the nitrogen cylinder to amidite bottles No 1 2 3 4 and No 6 7890X Air Switch On and Off the valve from the nitrogen cylinder to amidite bottles No 7 8 9 0 and X Lid OnOff Power on and off...

Страница 7: ...r priming CapA Opens Cap A valve for priming CapB Opens Cap B valve for priming Deb Opens Deb valve for priming Sol Opens Acetonitrile valve for priming ValveTime Increase opening time with 5 ms Valve...

Страница 8: ...m email karl ross dk p 8 3 4 Get Data Get data Imports data Prime Opens for Priming section 3 3 Manual control Opens for Checkpoints section 3 2 Delete Oligo Opens for removing single syntheses sectio...

Страница 9: ...is Prime Opens for Priming section 3 3 Stop Synthesis Stops the synthesis Pause Pause in the synthesis Delete Oligo Opens for removing single oligos in the synthesis section 3 6 Trityl Check The synth...

Страница 10: ...orgs Vej 12 DK 2000 Frederiksberg Tel 45 321 322 00 www oligomaker com email karl ross dk p 10 3 6 Delete Oligo Delete oligo 1 Enter the location of the oligo by typing the specific number of the olig...

Страница 11: ...gents designed for oligonucleotide synthesis are attached to OligoMaker Using a positive pressure less than 1 bar of Nitrogen or Argon the reagents are distributed to the columns through the valves an...

Страница 12: ...mended independent exhaust ventilation OligoMaker cannot start unless the front Lid is in place and the Reaction Block is filled with 12 columns When the tubing from the reagents are rinsed priming th...

Страница 13: ...goMaker ApS 8 Installation On site requirements A ventilated hood or similar connection to power 115 220 volts and connection to Nitrogen or Argon bottle at around 3 bar A valve should be nearby for p...

Страница 14: ...to waste container placed in ventilated area When you have placed the instrument in the selected location placed the vacuum pump and the Gas cylinder Nitrogen or Argon you can cut the tubing into 3 p...

Страница 15: ...on the floor below the synthesizer When you have decided the best location for your pump you can cut the tubing in the appropriate length You should connect the tubing to the in let on the pump When...

Страница 16: ...l others The manometers are situated on top of the instrument The vacuum manometer to the left shows the vacuum when the reagents are drained through the columns OligoMaker may only be started when th...

Страница 17: ...g to the real use adjusted on Synthesis Report delivered seperately On the Opening screen press Prime on the display Make sure you have activated the pressure to be able to prime Activate the pressure...

Страница 18: ...o push Prime and Prime all Pushing the Quit button terminates the process Holder in Priming Position If the reagent is not supplied during priming the tube or the valve must be inspected and if necess...

Страница 19: ...ick in the OligoMaker and select Get Data to transfer the sequence to the instrument All starting bases on the Synthesis Report is compared and reviewed If universal support is used this step can be s...

Страница 20: ...el or similar application and paste your sequences into the file The OM data file must be saved as a txt file in Excel For each run you can change the synthesis settings according to the guideline bel...

Страница 21: ...and the OligoMaker Opening time for the valves during the synthesis run For each reagent you can have individual opening times All times are calculated in msec All numbers shown have been used on a de...

Страница 22: ...use or finished in synthesis an extra time is added to the vacuum pump If you only have 1 or 2 active oligos left in a run they will require longer vacuum time to drain The vacuum time for each reage...

Страница 23: ...ines or delete lines if required The synthesizer will run trough this protecol for each cycle The waiting time before the vacuum sec Obsolete value not used cannot be deleted Please note During the sy...

Страница 24: ...45 321 322 00 www oligomaker com email karl ross dk p 24 Copy your sequences from your database and paste them into the OM data protocol Select DMT on or off On will leave final DMT group on for post...

Страница 25: ...r ethyl acetate NOT ethanol using for example a toothbrush 2 Priming of reagents before starting a synthesis 3 Check the tubing and the valves for leakage 4 Clean the top of the reaction block with a...

Страница 26: ...eorgs Vej 12 DK 2000 Frederiksberg Tel 45 321 322 00 www oligomaker com email karl ross dk p 26 12 Service 12 1 Service holder position Service Holder Position Lift the valve manifold and place it in...

Страница 27: ...2 2 Removing the U pin Removing the U pin When replacing a leaking valve place the service holder on the OligoMaker Tighten the two screws which are placed behind the service holder Place the Valve Ma...

Страница 28: ...mmed and pinched between the Alu cover and the Peek manifold Finally tighten the two screws Place the Valve manifold in the priming position and check if the new valves are working well Operators are...

Страница 29: ...M O ring 45 Kalrez O ring bottle neck 45 400 15 OM O ring I O ring inner drain 16 OM O ring O O ring outer drain 17 OM O ring RP O ring reaction plate 18 OM O ring Friction RP Upper Low Friction Ring...

Страница 30: ...4 x 11 46 OM BM 1 12 Feed Manifold 1 x 12 47 OM BM 2 12 Feed Manifold 2 x 12 48 OM BM 4 12 Feed Manifold 4 x 12 49 OM PM 4 4 Peek manifold 4 to 4 50 OM PM 2 4 Peek manifold 2 to 4 51 OM PM 1 4 Peek ma...

Страница 31: ...rk Tel 45 321 322 00 VAT no DK12783388 www oligomaker com Machine DNA Synthesizer X12 Serial No XX XX 19 2019 Manufacturing Year 2019 Machinery Directive ie Council Directive 2006 42 EC of 17 5 2006 o...

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