48
UNO-TS software Active Message
Control Software
Active
Display buttons
Disabled
Set Point 37.0°C
Base
37.0
HM 37.0
Lid
37.0
OBH 37.0
If a Function Button is pressed
when UNO-TS Software is
communicating with the Unit
2 seconds
Figure 40. Display when UNO-TS software is active.
When UNO-TS software is active, Menu navigation is disabled
14
Objective Heater Manual Calibration
Carefully read the instructions given in this paragraph and in paragraph 13.6 before
starting the Objective Heater Manual Calibration.
The goal of this procedure is to adjust the controlled Objective Heater temperature, manually
changing its offset; while maintaining the sample at the desired temperature (e.g. 37.0 °C)
once the oil immersion objective is in contact with the bottom of the well. This can be achieved
measuring the sample temperature with the Fine Gauge Thermocouple during steady state and
estimating the offset values, as follows:
1.
Plug the green Fine Gauge Thermocouple into the port labeled “Reference Temperature”
located in the rear panel of the Control Unit, as illustrated in Figure 14.
2.
Insert the Fine Gauge Thermocouple into the chamber by threading the wire through
one of the screw holes on the chamber riser.
3.
Secure the end of the Fine Gauge Thermocouple to the bottom of a Petri dish using
some tape making sure to avoid covering the head of the probe (see Figure 16).
4.
Fill the Petri dish with distilled water to a level sufficient to fully immerse the Fine Gauge
Thermocouple. This is a Reference Well simulating experimental conditions. Place the lid
