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  H301-PI-736-ZR1S/ZR2S 

User Manual 

   

REV 02

 

 www.oko-lab.com 

  

 

Pa

ge

6

 

5.

 

Connection of the Gas Supply 

A single silicon tubing carries output gas from the Okolab Gas Controller to the H301-PI-736-ZR1S/ZR2S. Silicon tubing 

connects to a gas input - brass opening - located on a corner of the H301-PI-736-ZR1S/ZR2S. See Figure 6. Connect by 

gently pushing silicon tubing onto brass opening. 

Gas Input

 

Figure 6.Connection with gas supply. 

6.

 

Working with Perfusion 

The  Chamber  Riser  included  with  H301-PI-736-ZR1S/ZR2S  features  12  perfusion  holes  for  the  insertion  of  perfusion 

tubing up to 2.5 mm in outer diameter. Small screws plug the perfusion holes when not in use. (

Grub screws M3x6

). 

Remove small screws as needed before introducing perfusion tubing.  

Figure 7 shows location of perfusion holes. 

1.Frontal view

2.3D view

Perfusion dedicated opening

 

Figure 7. Perfusion 

Содержание H301-PI-736-ZR1S

Страница 1: ...er Manual REV 02 www oko lab com Page 1 H301 PI 736 ZR1S ZR2S Compatible with the following Z piezo stages Compatible with the following Okolab Controllers PI P 736 ZR1S ZR2S H301 T BL PLUS UNO COMBINED CONTROLLER H401 T DUAL ...

Страница 2: ...onents and dimensions 3 2 Insertion of the Sample Feedback Temperature Sensor 4 3 Working with 35 Petri Dish 4 4 Working with 1 x3 and 1 x2 chamber slides 5 5 Connection of the Gas Supply 6 6 Working with Perfusion 6 7 Connection of the Chamber with Z piezo stage 7 8 Cleaning 8 ...

Страница 3: ...ating and embedded temperature sensor Chamber riser It is a removable frame increasing the height of the chamber from 20 to 25 mm Distance between focal plane and upper side of the glass lid without the chamber riser Distance between focal plane and upper side of the glass lid with the chamber riser Chamber top view Lid Chamber Riser Chamber Main Body Sample Holder Ordered Separately Figure 1 H301...

Страница 4: ...01 PI 736 ZR1S ZR2S chamber riser see Figure 2 Frontal and 3D views 1 Frontal view 2 3D view Temperature Sensor dedicated opening Temperature Sensor dedicated opening Figure 2 Insertion of the temperature sensor inside the chamber 3 Working with 35 Petri Dish Flat springs prevent movement of 35 mm Petri dishes inside of the sample holder See Figure 3 1 2 3 A B Figure 3 Plate adapter for 35mm Petri...

Страница 5: ...orking with 1 x3 and 1 x2 chamber slides Flat springs prevent movement of 1 x3 and 1 x2 chamber slides inside of the sample holder A A 1 2 Figure 4 Sample holder for 1 x3 chambered cover glass holder 2 1 A A Figure 5 Sample holder for 1 x2 chambered cover glass holder ...

Страница 6: ...y gently pushing silicon tubing onto brass opening Gas Input Figure 6 Connection with gas supply 6 Working with Perfusion The Chamber Riser included with H301 PI 736 ZR1S ZR2S features 12 perfusion holes for the insertion of perfusion tubing up to 2 5 mm in outer diameter Small screws plug the perfusion holes when not in use Grub screws M3x6 Remove small screws as needed before introducing perfusi...

Страница 7: ...ct the chamber with Z piezo stage 1 Place the sample holder on the stage and tighten the four M2x3 Pan head screws as shown in Figure 8 Image 1 2 Place the chamber main body on the stage and tighten the two M2x3 Pan head screws as shown in Image 2 and 3 Figure 8 3 Place the lid on the chamber as shown in Image 3 of Figure 8 1 2 4 3 Screw M2x3 Screw M2x3 Figure 8 How to assemble the chamber to the ...

Страница 8: ...e chamber cools down To clean the body and the glass lid of the chamber wipe with a soft micro fiber cloth For stubborn smudges you can damp the soft micro fiber cloth with ethyl alcohol product code UN1170 Do not put any liquid di rectly on the chamber While cleaning the glass lid do not apply strong force to the surface of the glass lid because it can be damaged H301 PI 736 ZR1S ZR2S_User Manual...

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