NuAire NU-8600, Руководство по эксплуатации и обслуживанию, Страница: 3 / 53

OM0253 Page 3 of 53
Rev 6 November/2018
Water Jacket
Invitrocell CO
2
Incubator
Model: NU-8600, NU-8600E, NU-8631, NU-8631E
Operation and Maintenance Manual
1.0 General Description
The NuAire Water Jacketed Incubator has been designed to provide a reliable controlled In-vitro environment for optimum
tissue cell culture growth. The chamber also provides an environment for the storage and preservation of gametes and animal
tissue cell cultures intended for research at or near body temperature.
There are five parameters that contribute to optimum growth conditions, they are:
Humidity
Precise temperature control
Precise CO
2
control
Sterility
Reliability
Like all NuAire equipment, this Incubator has been designed to provide the highest quality standards of performance with
matching computer technology, precise temperature control, and CO
2
gas control system combining state-of-the-art technology
with years of design, quality, and manufacturing experience.
1.1 Extra Large (18 to 20 gallon) Water-Jacket - Each Chamber
The outer stainless steel wall is lined with a space-age insulation providing a R5.0 rating, minimizing heat loss. The large 20
gallon (75.7 liters) Water-Jacket utilizes water, one of nature’s best "sinks" of heat. Its high capacity to hold heat makes it
the ideal medium to surround a chamber in order to obtain temperature uniformity. In fact, the ability of materials to hold
heat, called the specific heat, uses water as the comparative standard. The large water-jacket surrounding the chamber
permits the water to circulate within the jacket, producing a temperature uniformity of ± 0.2 ° C. The larger the mass, the
less susceptible the environment within the chamber is to environment fluctuations outside. It also adds cabinet stability
for the growth of vibration sensitive cells.
1.2 Incubator Closed loop chamber sample circulation system & HEPA Filter
A continuous operating air pump draws environmental sample from the chamber and circulates it through a HEPA filter
capsule then through sensors that measure CO
2
volume (as a percentage) in the chamber for monitoring and control.
1.3 Incubator Control Electronics
The NuAire Incubator Control Electronics is a state-of-the-art microcomputer based control system specifically designed to
service the precise control requirements of the chambers environment, providing optimum programmable conditions for
culture growth. The microcomputer is “user-friendly” and provides a 5x7 LCD screen which provides operating control
parameters, status indicators, additional key operational parameters, and an imbedded touch panel to permit efficient
operator entry of data.
The microcomputer is supported with Read Only Memory (ROM) containing executable software, Random Access Memory
(RAM) for temporary storage, and Electronically Erasable Programmable Read Only Memory (EEPROM) for control set
points and parameters. The EEPROM provides for indefinite storage of these values during periods of power off or power
interruption (power fault tolerant).
1.4 Incubator CO
2
Control
The NuAire direct heat Incubator incorporates a microprocessor-based, non-dispersive infrared CO
2
induction sensor.
The amount of energy received at the detector is an approximate logarithmic function of the CO
2
concentration in the gas
between source and detector. The wavelengths used are absorbed only by CO
2
making the measurement insensitive to
other components, such as water vapor. Detector linearization is performed with 32 bit digital accuracy. Advanced design
provides a very stable output minimizing drift and requiring less frequent calibration. The output is digital, alleviating errors
brought about by analog signals. Calibration of this control is accomplished by a zero & span calibration done on the sensor
and a display off- set calibration. These calibrations are accessible through the NuTouch LCD located on the front of the
unit.