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PDES-01DM User Manual 

version 1.3 

 

page 13 

4.2. Operation 

 

 

Connect the gas source (max. 5 bar) to the INPUT connector at the front panel.  

Important

: Do not connect sources of OXYGEN or COMBUSTIBLE gases!! The gas source 

MUST be dry and oil free!! Please use a degreaser and filter the gas source by a 5 µm filter 
element. 

 

Connect the injection pipette to the OUT connector at the front panel  

or 

 

connect the 

micro

JECT pipette holder to the two 

micro

JECT connectors at the front panel 

(#

6

 and #

7

, Figure 5), and connect your ejection pipette to the 

micro

JECT (#

1

, Figure 6). 

 

 

If a low "retain" pressure (analogous to the "retaining" current used in iontophoretic 
systems) is required, connect the “retain” pressure source to EXHAUST at the front panel. 

 

Turn on pressure and adjust the pressure to the desired value using the pressure regulator 
(#

5

, Figure 5). 

 

Connect a timing unit to the TTL INPUT connector (#

11

, Figure 5) at the front panel 

or 

connect a pushbutton to the REMOTE CONTROL connector (#

10

, Figure 5) at the front 

panel 

 

The LED is green when no ejection occurs and red when the pressure is applied to the 
pipette. 

 

Select the operation mode using the rotary switch (#

12

, Figure 5) at the front panel. 

 

In the CONT. position positive pressure is applied to the pipette continuously. 

 

In the EXTERN position the duration of the application is determined directly by the 
duration of the TTL signal or the time the pushbutton is pressed. 

 

In the TIMER position the duration is preset by the timer control (#

14

, Figure 5) and 

time base (#

13

, Figure 5) of the PDES. Application is started with a TTL signal 

connected to TTL INPUT (#

11

, Figure 5) or by pressing the pushbutton connected 

to #

10

, Figure 5. In position OFF of the timer control for pressure ejection is disabled. 

 

In the OFF position the PDES module is turned OFF, the valve is closed. LED (#

15

Figure 5) is also off. The source pressure value is still shown at the manometer #

1

Figure 5, but opening of the valve is disabled in OFF position.  

 

Hint

: Ejection time can be monitored by recording the signal at #

8

, Figure 5! 

 
 

 

 

Содержание PDES-01DM

Страница 1: ...PDES 01DM PNEUMATIC DRUG EJECTION MODULE WITH ANALOG TIMER FOR EPMS 07 SYSTEMS VERSION 1 3 npi 2016 npi electronic GmbH Bauhofring 16 D 71732 Tamm Germany Phone 49 0 7141 0730230 Fax 49 0 7141 9730240...

Страница 2: ...Grounding 6 EPMS 07 EPMS 03 6 EPMS E 07 6 2 8 Technical Data 6 EPMS 07 EPMS E 07 and EPMS H 07 6 EPMS 07 and EPMS H 07 6 EPMS E 07 6 EPMS 03 6 3 PDES System 7 3 1 System Description 7 3 2 PDES 01AM C...

Страница 3: ...LECTRICITY Electronic equipment is sensitive to static discharges Some devices such as sensor inputs are equipped with very sensitive FET amplifiers which can be damaged by electrostatic charge and mu...

Страница 4: ...ront covers Figure 1 Left front view of empty EPMS 07 housing In order to avoid induction of electromagnetic noise the power supply unit the power switch and the fuse are located at the rear of the ho...

Страница 5: ...ernal power supply PWR 03D is capable of driving up to 3 EPMS E housings Each housing is connected by a 6 pole cable from one of three connectors on the front panel of the PWR 03D to the rear panel of...

Страница 6: ...d to the SYSTEM GROUND SIGNAL GROUND on the rear panel of the instrument see Figure 4 The chassis can be linked to PROTECTIVE EARTH by connecting it to the PWR 03D with the supplied 6 pole cable and b...

Страница 7: ...front panel If the internal timer is used the eject time is preset by a control and the time base can be selected by a toggle switch The operational status is indicated by a red green LED The pressure...

Страница 8: ...utton External valve slow switch time 10 ms 3 3 Description of the Front Panel Figure 5 PDES 01DM front panel view the numbers are related to those in the text below 1 Manometer Digital display for th...

Страница 9: ...re is indicated by the manometer 1 6 OUT connector Connector for connecting the injection micropipette or for connecting the tubing to external valve e g the micropipette holder microJECT The EJECT pr...

Страница 10: ...x1s x0 01s x0 01s switch Switch to set the time base of the timer 14 range x10 s x1 s x0 1 s or x0 01 s The total ejection time is the reading of this switch multiplied by the reading of the timer po...

Страница 11: ...e The valve which is controlled by the PDES is located on the remote valve plate see Figure 6 Tubing and cable of the external valves are connected at the front panel of the PDES system refer to chapt...

Страница 12: ...y not connected Figure 7 microJECT 1 pipette connector 2 connector for EJECT pressure 3 connector for EXHAUST pressure optional 4 electrical connection Important In experiments with high pressures 1 b...

Страница 13: ...connect a pushbutton to the REMOTE CONTROL connector 10 Figure 5 at the front panel The LED is green when no ejection occurs and red when the pressure is applied to the pipette Select the operation m...

Страница 14: ...PDES 01DM User Manual version 1 3 page 14 Figure 9 Operation modes of the PDES 01DM...

Страница 15: ...iol 587 5859 5877 Lin B J Chen T W Schild D 2007 Cell type specific relationships between spiking and Ca2 i in neurons of the Xenopus tadpole olfactory bulb Journal of Physiology 582 163 175 Marandi N...

Страница 16: ...kidney 293 cells and Xenopus Laevis oocytes J Neurosci 19 7426 7433 Fast drug application with microJECT EHM Courjaret R Troger M Deitmer J W 2009 Suppression of GABA input by A1 adenosine receptor a...

Страница 17: ...red light valve is closed in EXTERN TIMER CONT operation mode green light valve is open Off no light valve is closed Mode selection three position toggle switch TIMER CONT EXTERN Minimum valve switch...

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