4
Unpacking
1. To remove the Lid, hold the device firmly and slide the Lid as indicated on the
top.
2. Pull out the blue Wedges to release the Inner Chamber (Cathode Buffer
Chamber Frame).
3. Take Out the Inner Chamber.
Assembly and Operation
Caution: Electrophoresis operates at a high voltage and Gel Cassetes are fragile by
nature. Some protective measure like wearing gloves should be taken when handling
FastGene
®
Protein Electrophoresis Chamber.
1. Ensure that the power supply is turned off and the Lid is not connected.
2. Ensure the Gaskets are set on the Inner Frame (Cathode-Buffer Chamber
Frame). Otherwise place the Gaskets on the grooves of the Inner Frame and
press the Gaskets so that they are fixed firmly into the grooves.
3. Pour the buffer (approximately 200 ml) in the Outer Anode Buffer Chamber up
to around 4 cm from the bottom.
4.
Take two IDMax™ Precast Gel Cassettes and remove the combs gently
. Take the
Inner Frame and position the Gel Cassettes so their bottom is covering all the
“white” part of the gasket
and the loading opening facing toward the inner
frame.
If using only one gel at a time, use one
Dummy Gel Cassette
instead of the
second IDMax™ Precast Gel C
assette. Place the Dummy Cassette upside down,
and the loading opening outside of the central reservoir, so it will not allow
contact between the 2 buffer reservoirs.
