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Points for Attention to Operation 

1.

 

The Spectro 96  Fluorospectrophotometer  is  suitably  used

  in  a  laboratory  room  to  perform 

analytical  measurements.  If  the  instrument  needs  to  be  used  at  a  worksite,  the  working 
environment of the worksite should accord with the demands of the laboratory environment 
basically. 

2. If the user needs to move the instrument to other working site for analytical  measurements, 

please use original packing materials on moving. 

3.  Be  sure  to  turn  on

off  the  power  of  the  instrument  according  to  the  operating  procedure. 

When starting up the instrument, please turn on the power source of the xenon lamp first. 
Then turn on the power source of the spectrophotometer main unit after the xenon lamp is 
lit.  When  shutting  down  the  instrument,  please  turn  off  the  power  source  of  the 
spectrophotometer  first  and  then  the  power  source  of  the  xenon  lamp.  Otherwise,  the 
instrument may not work normally. After the xenon lamp is lit, it is necessary to wait for 30 
minutes over till the lamplight becomes stable. After the power source of the xenon lamp is 
turned  off,  please  wait  for  60  seconds,  and  then  turn  on  the  lamp  if  the  lamp  needs  to  be 
used again.   

4.  When  the  power  source  of  the  xenon  lamp  is  turned  on  but  the  lamp  fails  to  be  lit  and  a 

squeaking  sound  of  high  frequency  or  a  cracking  sound  of  sparking  is  produced 
continuously,  please  turn  off  the  power  source  of  xenon  lamp  immediately  and  wait  for 
several seconds, then turn on the lamp again. Because the life of the xenon lamp is closely 
related  to  the  number  of  on-off  of  the  lamp  power  source,  the  lamp  should  avoid  being 
turned on/off repeatedly under a high voltage and the unnecessary number of on/off of the 
xenon lamp power source should be cut down as far as possible. 

5.

 

When the instrument is working, the surface temperature of the heat dissipation opening at 
the top of the left back side of the instrument (xenon lamp position) becomes very high. Be 
sure not to touch the surface of the heat dissipation opening with hands in order not to be 
burnt. The air around the heat dissipation opening should be kept circulating. 

6.

 

After each startup of the instrument, please first confirm that the heat dissipation fan at the 
side  of  the  instrument  runs  normally  in  order  to  ensure  normal  work  of  the  instrument.  If 
the user finds anything wrong with the heat dissipation fan, the instrument should be closed 
down for an overhaul. 

7.

 

In order to protect the photomultiplier when PMT level is higher (>6), do not let high light 
pass into the sample compartment. When a sample of unknown concentration is measured, 
PMT level should be set from low grade to high grade (1-17) gradually. 

8.

 

After  PMT  level  is  adjusted,  the  original  set  for  zero-point  of  fluorescence  should  be 
checked and note that zero-point adjustment may be needed again. 

9.

 

When  and  only  when  a  computer  error  is  due  to  the  operator¹s  false  operation  or  other 
interferences,  the  power  switch  of  the  spectrophotometer  main  unit  should  be  turned  off 
immediately.  When  the  software  cannot  be  operated,  please  use  task  manager  to  end  the 
“f96pc.exe” process, then restart the software and the instrument. 

10.

 

Within the monochromator the tightened screws must not be loosened. The optical devices 
and the working environment of the instrument should be kept clean. 

11.

 

When  opening  the  cover  to  inspect  the  instrument,  be  sure  to  turn  off  the  power  source. 
Particularly  after  the  upper  cover  of  the  instrument  is  removed,  as  there  are  electrical 
appliance parts with high voltage located at the left back of the instrument base, be sure not 
to contact these parts while the power source is still turned on. 

 

 

INSTRUCTION MANUAL    FOR SPECTRO 96 FLUOROSPECTROPHOTOMETER 

Содержание SPECTRO-96

Страница 1: ...ASE READ THIS MANUAL CAREFULLY BEFORE OPERATION 3 Hagavish st Israel 58817 Tel 972 3 5595252 Fax 972 3 5594529 mrc mrclab com MRC VER 01 5 12 Operation Manual for FLUORESCENT SPECTROPHOTOMETER SPECTRO...

Страница 2: ...ument should have a basic knowledge of using optical instruments and a basic knowledge of molecular fluorescence spectroscopy The user whose instrument is equipped with a computer also should have a c...

Страница 3: ...tially hazard to the electrical appliances through the operation and indicates that a qualified professional man is needed to deal with such a hazard according to the corresponding procedures This lab...

Страница 4: ...as possible 5 When the instrument is working the surface temperature of the heat dissipation opening at the top of the left back side of the instrument xenon lamp position becomes very high Be sure no...

Страница 5: ...on of Startup 17 2 2 1 Startup of the Instrument 17 2 2 2 Confirmation of Heat Dissipation Fan Running 17 2 2 3 Initialization 17 Table 1 2 Fluorospectrophotometer Initialization List 18 2 3 Measureme...

Страница 6: ...Time Scan 37 6 2 3 The Interface of Quantitative Analysis 38 7 Operation of Wavelength Scan Mode Connected with a Computer 39 7 1 The Interface of Wavelength Scan Mode 39 7 1 1 The Functions of Short...

Страница 7: ...3 9 2 3 Zero point Adjustment 53 9 2 4 Standard Sample Setting 54 9 2 5 Measurement of Standard Samples 54 9 2 6 Method of Undetermined Coefficients 55 9 2 7 Measurement of Samples 55 9 2 8 Set Sample...

Страница 8: ...e Complete Instrument Operating Panel Fig 1 1 SPECTRO 96 Fluorospectrophotometer Main Unit Heat Dissipation Opening at the Top of the Instrument Heat Dissipation Fan Power Line Lid of Sample Compartme...

Страница 9: ...it a cell holder used to hold a sample cell Power Switch used to turn on off the power source of the instrument Fuse Holder used to mount a fuse Power Socket used to interconnect a feed cable Xenon La...

Страница 10: ...9 Main Components 1 Xenon Lamp Fig 1 3 Xenon Lamp and Lamp Holder Line Xenon Lamp The lightening part Line Lens INSTRUCTION MANUAL FOR SPECTRO 96 FLUOROSPECTROPHOTOMETER...

Страница 11: ...ilter center wavelength 365 nm 2 Sample Compartment Fig 1 4 Sample Compartment and Quartz Sample Cell 10 mm 3 Filter Filter Position Sample Cell Position INSTRUCTION MANUAL FOR SPECTRO 96 FLUOROSPECTR...

Страница 12: ...ectrop hotometer Main Unit 1 Mode key 2 Print key 3 Adj 0 key 4 Normalize key 5 Display Window 2 5 4 3 1 1 2 Operating Panel of the Instrument 1 Appearance and Performances of the Instrument INSTRUCTI...

Страница 13: ...s of the Instrument Xenon Lamp Power Source of Xenon Lamp Excitation Filter Sample Cell Emission Monochromatoraton Photomultiplier Preamplifier Monolithic Processor Serial Interface USB Communication...

Страница 14: ...13 Optical System Fig 1 8 Optical System Arrangement of SPECTRO 96 Fluorospectrophotometer INSTRUCTION MANUAL FOR SPECTRO 96 FLUOROSPECTROPHOTOMETER...

Страница 15: ...1 Measuring with a single instrument By using the functions of the keys and the functional indicator lights on the instrument panel read the measured data on the display window 2 Measuring with an on...

Страница 16: ...racy within 1nm Wavelength repetition 0 5nm Emission spectrum bandwidth 10nm S N ratio S N 150 Using 1cm quartz sample cell measure the signal noise ratio of Raman spectrum of water Check utmost 1 10...

Страница 17: ...lue use Mode key to cancel current value and return the set value 2 Print Key The Print key can print the current value if a dedicated serial interface printer is installed in Fluorescence Value Mode...

Страница 18: ...e unnecessary number of turning on off the xenon lamp should be cut down as far as possible and the xenon lamp should avoid being turning on off repeatedly under a high voltage 2 2 2 Confirmation of H...

Страница 19: ...ed Photomultiplier tube error PMT Module error Self checking Motor Module Motor Module initialized Motor error Motor driver board error Self correcting Wavelength correcting The value from bottom to t...

Страница 20: ...ethods that is multipoint method of standard sample and single point method of standard sample The two operating methods are as follows 1 Measuring procedures by Multipoint Method of Standard Sample P...

Страница 21: ...on of maximal concentration into sample cell Then press Mode to enter PMT level adjust Current PMT Level shows the current level The higher the level is the more sensitive it will be Use Adj 0 key or...

Страница 22: ...ument Turn off the power source of the Fluorospect rophotometer main unit first and then the power source of the xenon lamp The order is reverse to that of startup If in on line mode close the softwar...

Страница 23: ...violet radiation should not be contained in the atmosphere 3 1 2 Working Table The instrument should be placed on a sturdy and level table or the like avoiding strong vibration direct sun light and d...

Страница 24: ...g marks of impact on the packing please contact the transport insurance department 3 2 2 Unpacking for Counting Unpack at the position of adhesive seal tape Take out the Fluorospectrophotometer main u...

Страница 25: ...ference filter 25nm Quartz sample cell 10mm Glass sample cell 10mm Fuse 2A 5A USB wire Spare parts Purchased by choice Power line Microcomputer main unit Display device Dedicated serial interface prin...

Страница 26: ...ument should accord with that of the local 3 3 3 Connecting the Power Cord Place the Fluorospectrophotometer main unit and the computer or the printer on a sturdy working table and they should be 10cm...

Страница 27: ...d 200 Find the wavelength value max of the maximum fluorescent intensity at about 415nm For more information of adjustment please turn to 2 3 2 Then observe for 2 minute at the wavelength max Record t...

Страница 28: ...mputer Finish the measurement for Raman Spectral Peak of water by using wavelength scan and time scan in the computer software The theory is the same as Section 3 4 1 Part 1 The use of software please...

Страница 29: ...do such work The concrete method and the correcting procedures will be briefed through training The wavelengths of spectral lines of a mercury arc lamp are listed in the Table 1 1 and the spectrogram...

Страница 30: ...ning at the top of the instrument It is unsuitable to stack other articles nearby Note The temperature at the heat dissipation opening is very high Be sure not to touch for fear of being burnt 3 Keep...

Страница 31: ...non lamp fails to be lit and a squeaking sound of high frequency or a cracking sound of sparking is continuously produced please turn off the power source of the xenon lamp immediately Several seconds...

Страница 32: ...over board on the excitation part from the top left of the knurled screw 3 Take out the excitation part 4 Remove the front pressing ring of the excitation part anticlockwise 5 After that hold the both...

Страница 33: ...32 Part Two Operation Instruction for the Instrument Connected with a Computer INSTRUCTION MANUAL FOR SPECTRO 96 FLUOROSPECTROPHOTOMETER...

Страница 34: ...wo serial ports of the computer should run normally and no interruption conflict occurs in the system Image resolution is more than 800 600 and color enhancement is more than 16 bit 5 1 2 Software Req...

Страница 35: ...e is The Software Package of Fluorospectrophotometer You may click NEXT button directly or change the item name then confirm the dialog boxes of all above steps If hoping to amend click Back button or...

Страница 36: ...n line condition 3 Initialization Run Spectro96 Software The software will check the connection and starts initialization For more information please turn to 2 2 1 On completion of initialization the...

Страница 37: ...36 Fig 2 1 Interface of Wavelength Scan Fig 2 2 Switchover of Working Mode PMT Storage EM Wavelength Wavelength Scan Fluor Fluor INSTRUCTION MANUAL FOR SPECTRO 96 FLUOROSPECTROPHOTOMETER...

Страница 38: ...and can also be used to study the emitting behavior of fluorescent molecules in solutions The interface of wavelength scan mode is displayed as shown in Fig 2 1 6 2 2 The Interface of Time Scan Time...

Страница 39: ...ncentration of the component to be determined is acquired K in the formula is a constant related to the test conditions and it can be acquired by measuring the fluorescent emission intensity of a stan...

Страница 40: ...ig 2 5 Shortcuts in the Interface of Wavelength Scan of Fluorescence Spectrum 7 1 1 The Functions of Shortcuts at the Bottom of the Screen Scan is the button for starting After you click it scanning a...

Страница 41: ...uantitative analysis mode Parameter button means to set scan parameters Restore Parameter button means to restore the spectrum of current channel to its original status Smooth button means to carry ou...

Страница 42: ...rameter the interface of wavelength Scan Parameter will appear as shown in Fig 2 6 Fig 2 6 Interface of Wavelength Scan Parameter 1 EM Start Set the start wavelength of scan 2 EM End Set the end wavel...

Страница 43: ...wavelength scan Batch will show all data after the wavelength scan 11 Slit Its value is unchangeable and it has a bandwidth of 10nm Fig 2 7 Interface of Wavelength Scan Parameter Replicate Scan Setti...

Страница 44: ...tly use the mouse to drag the spectrum on the screen The method is to drag in the direction from the top left side of the spectrum to the bottom right side of the spectrum If you want to return the en...

Страница 45: ...between channels Fig 2 10 Interface of Arithmetic Operation 7 Sigma After clicking this item input Lambda Start and Lambda End and then click the Sigma button You will get the integral value of fluor...

Страница 46: ...he spectrum of one channel into another channel 4 Save Spectrum Save the selected spectrum and the file name onto the hard disc 5 Save Spectrum As Save the spectrum onto the hard disc After a dialog b...

Страница 47: ...ange the background color of the working window 3 Set Background Change the background color of scanning window 4 Set Netware Change the net type of the scanning window 5 Export Window as Export the c...

Страница 48: ...nged to the interface of time scan See Fig 2 3 The time scan interface is the same as that of wavelength scan interface except for the unit of X axis The functions of the shortcuts are also the same F...

Страница 49: ...rum The software will automatically add the current time of scan after the filename 13 Auto Saved Direction Set the autosave position in your computer 8 1 3 Spectrum Processing The functions and the o...

Страница 50: ...tering the interface of Quantitative Analysis Above the interface of quantitative analysis on the top of the screen the first row is menu bar The second row has 15 icons The screen shows the Current W...

Страница 51: ...will show Icons are arranged in order from left to right The functions are described bellow Set QA Parameter button This feature has not been activated yet Set Working Curve Parameter button means to...

Страница 52: ...Method of Undetermined Coefficients The method by which an equation of working curve is created through measuring standard samples is called Method of Undetermined Coefficients For more details refer...

Страница 53: ...vious data of standard solutions so as to make a direct measurement for samples of the same kind After clicking Build Curve in Data Processing menu you may see the working curve the equation and the c...

Страница 54: ...9 2 2 PMT Setting There are 17 grades 1 17 Default PMT level is 1 Generally adjust PMT level by using the highest concentration of the solution Adjust the fluorescence value to make sure it is stable...

Страница 55: ...is See Fig 2 20 Fig 2 20 Dialog Box of Set Standard Samples Note The parameter setting of standard samples must follow the order of concentration from low to high The names of standard samples includi...

Страница 56: ...urve is created through measurement of standard samples is called Method of Undetermined Coefficients If an equation of working curve is known you may select Input Coef As for the measuring of samples...

Страница 57: ...middle as shown in Fig 3 1 after inputting Fig 3 1 Screen Display after Inputting Fluorescent Intensity and Concentration 2 Enter the Chart Wizard System 1 Preview Generated Graphics Click the Chart...

Страница 58: ...The Standard Curve Plotted after Data Processing With Excel 2000 Software in Measurement of Riboflavin by fluorophotometry y 437 14x 0 2762 the fitted equation correlation coefficient R2 0 9997 4 Meas...

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