VersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide
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0112-0126 B
SpectraMax Plus 384 Specifications
Performance specifications for cuvette reads apply to aqueous solutions that have solute
molal concentrations less than 0.4 M. When you apply pathlength compensation to plate
absorbance measurements, agreement with cuvette absorbance measurements for the
same solution requires that the solution volume in the plate well is between 100 μL and 300
μL.
Item
Description
Wavelength range
190 nm to 1000 nm
Wavelength selection
Monochromator tunable in 1 nm increments
Wavelength bandwidth
≤ 2.0 nm full width half maximum
Wavelength accuracy
±1.0 nm across wavelength range
Wavelength repeatability
±0.2 nm
Photometric range
0 to 4.000 OD
Photometric resolution
0.001 OD
Photometric accuracy linearity (plate),
0-2.0 OD
190-1000 nm < ± 1.0% and ± 0.006 OD
Photometric accuracy linearity (cuvette),
0-2.0 OD
190-1000 nm < ± 1.0% and ± 0.005 OD
Photometric precision (repeatability)
190-1000 nm < ± 1.0% and ± 0.003 OD
Stray light
≤ 0.05% at 230 nm
Photometric stabilization
Instantaneous
Photometric drift
None (continuous referencing of monochromatic input)
Calibration
Automatic before every endpoint read and before the
first kinetic read
Optical alignment
None required
Light source
Xenon flash lamp (5 Watts)
Average lamp lifetime
1 billion flashes
Illumination
Top down (plates); horizontal (cuvettes)
Photodetector
Silicon photodiode
Photometric Performance SpectraMax Plus 384
