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VersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide

0112-0126 B

Item

96-wells

Endpoint standard read

9 seconds single wavelength
19 seconds dual wavelength 425 & 650 nm

Kinetic read intervals

9 seconds minimum interval between reads single wavelength
1 column, 2-second minimum interval between reads
Single wavelength

Measurement Time (calibration off) SpectraMax 190

Item

Description

Plate: normal scan

33*K nm/min (8-well strip)

Plate: speed scan

135*K nm/min (8-well strip)

Scan Speed (*K = wavelength interval) SpectraMax 190

Item

Description

Read chamber

Isothermal when temperature regulation is not enabled

Range

4°C above ambient to 45°C when temperature regulation enabled.
The ambient temperature must be >20°C to achieve temperature
regulation at 45°C.

Resolution

± 0.1°C

Accuracy

± 1.0°C for plate chamber

Temperature uniformity
at equilibrium

± 0.5°C at 37°C

Chamber warm-up time

15–30 minutes (measured on air) after initiation of temperature
regulation

Temperature regulation

4 sensors

Drift

± 0.2°C (regulated)

Temperature regulation
diagnostics

Temperature regulation system is continuously monitored and updated

Evaporation

Plate lid required to minimize evaporative cooling

Recommended plate

Flat-bottom plates with isolated wells and lid

Temperature Regulation SpectraMax 190

Содержание versamax

Страница 1: ...0112 0126 B October 2018 SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC384 VersaMax Microplate Spectrophotometers User Guide...

Страница 2: ...any right and or license to use or permit others to use such manufacturers and or their product names as trademarks Each product is shipped with documentation stating specifications and other technic...

Страница 3: ...ter 4 Absorbance Read Mode 19 PathCheck Pathlength Measurement Technology 20 Read Types 24 Chapter 5 Maintenance 25 Cleaning the Instrument 25 Replacing Fuses 26 Before You Move the Instrument 28 Chap...

Страница 4: ...sed in a manner not specified by Molecular Devices the protection provided by the equipment might be impaired Warnings Cautions Notes and Tips All warning symbols are framed within a yellow triangle A...

Страница 5: ...ic Equipment WEEE Directive of the European Union It indicates that you must not discard this electrical or electronic product or its components in domestic household waste or in the municipal waste c...

Страница 6: ...y harmful When you use such materials observe the following precautions Handle infectious samples based on good laboratory procedures and methods to prevent the spread of disease Observe all cautionar...

Страница 7: ...red To prevent injury Never try to exchange labware reagents or tools while the instrument is operating Never try to physically restrict the moving components of the instrument Note Observe all warnin...

Страница 8: ...ndpoint at a single point in time Kinetic over a specified period of time Spectrum over a specified wavelength range The shake feature enables you to mix the contents of the wells in a plate before ea...

Страница 9: ...is not available for the VersaMax To run protocols that require advanced acquisition settings or to run Absorbance read mode Kinetic read type and Spectrum read type protocols you must connect the in...

Страница 10: ...settings to shake the plate linearly along the long axis at preset intervals to mix of the contents of the wells Endpoint read type Settings enable you to shake the plate for a definable number of sec...

Страница 11: ...s 384 where the instrument control panel displays the cuvette chamber temperature Use the SoftMax Pro Software to view the plate chamber temperature for the SpectraMax Plus 384 Typically the plate cha...

Страница 12: ...te to prevent evaporation of the sample The seal or lid also helps to maintain a uniform temperature It can take an hour or more for a prepared sample to equilibrate inside the plate chamber You can s...

Страница 13: ...omplete list of the package contents see the enclosed packing list The packaging is designed to protect the instrument during shipment Tape is placed on the cuvette door and the plate drawer to protec...

Страница 14: ...witch that is on the rear of the instrument is in the Off position 2 To use a computer to operate the instrument connect the appropriate end of the supplied USB cable to the USB port that is on the re...

Страница 15: ...the front of the instrument now faces you Note Ensure no cables run beneath the instrument 7 Remove the tape from the cuvette door on the SpectraMax Plus 384 8 Power on the instrument and wait for the...

Страница 16: ...d Analysis Software User Guide When you connect the instrument to a printer and operate it in stand alone mode the instrument control panel enables you to run basic Absorbance read mode protocols The...

Страница 17: ...nty SoftMax Pro Software Workflow For this workflow you connect the instrument to the computer on which you install the SoftMax Pro Software See the SoftMax Pro Data Acquisition and Analysis Software...

Страница 18: ...r 2 Use the instrument control panel to set the wavelength and temperature for the read Wait for the plate chamber or cuvette chamber to reach the set temperature 3 Use the instrument control panel to...

Страница 19: ...total quantity of light available Optical Density includes absorbance of the sample plus light scatter from turbidity and background You can compensate for background using blanks A blank well contain...

Страница 20: ...distance of the light through the sample depends on the volume This variable pathlength makes it difficult to do extinction based assays and makes it confusing to compare results between microplate re...

Страница 21: ...t concentration less than 0 5 M the Water Constant correction method is sufficient The Water Constant is determined for each instrument during manufacture and is stored in the instrument Cuvette Refer...

Страница 22: ...thlength Measurement Technology feature after the read Eliminating Pathlength Independent Component Raw OD measurements of plate samples include both pathlength dependent components sample and solvent...

Страница 23: ...highly colored in the upper visible spectrum might have absorbance that extends into the near infrared NIR spectrum and can interfere with the PathCheck technology Examples include Lowry assays molybd...

Страница 24: ...Sec Time to VMax and Onset Time Kinetic reads can be single wavelength or multiple wavelength readings The kinetic read type can collect data points in time intervals of seconds minutes or hours up to...

Страница 25: ...re applicable to do so If parts are returned they must be enclosed in a sealed plastic bag that states that the contents are safe to handle and are not contaminated WARNING BIOHAZARD Always wear glove...

Страница 26: ...rument requires periodic cleaning The frequency of cleaning depends on the cleanliness of the lab and could range from once a month to once every six months 1 Remove the plate or adapter from the plat...

Страница 27: ...at requires removal of a panel or cover or disassembly of an interior instrument component 1 Power off the instrument 2 Unplug the power cord from the power port 3 Use a small slot head screwdriver to...

Страница 28: ...following applicable decontamination procedures and packing instructions Note If you must store the instrument then store it in a dry dust free environmentally controlled area Packing the Instrument...

Страница 29: ...ory For assistance regarding warning messages contact your local Molecular Devices representative Error Code Classifications Not all error messages are listed here Error Code Ranges Possible Causes 10...

Страница 30: ...d not perform the command because it was busy doing another task 106 command invalid measurement in progress Instrument could not perform command because a measurement was in progress 107 no data to t...

Страница 31: ...ignoring flow control 203 serial port parity error Parity bit error detected with incoming serial data 204 serial port overrun error Caused by host computer sending too much data and ignoring the flow...

Страница 32: ...tte door being open 305 reference level saturation During a cuvette read could be due to cuvette door being open 306 plate air cal fail low light Minimum signal reference ratio not met during air cali...

Страница 33: ...error Plate air calibration data is unreasonable 504 NVRAM Carriage offset error Carriage offset data is unreasonable 505 NVRAM Stage offset error Stage offset data is unreasonable 506 NVRAM Battery T...

Страница 34: ...ting our customers with the highest level of technical service Our Support website www moleculardevices com service support has a link to the Knowledge Base which contains technical notes software upg...

Страница 35: ...optical performance Part Number Item Name Compatible Instruments 0200 6117 SpectraTest ABS1 Absorbance Validation Plate SpectraMax iD3 iD5 i3x i3 M2 M2e M3 M4 M5 M5e Plus 384 340PC 384 190 ABS ABS Plu...

Страница 36: ...maximum Wavelength accuracy 1 0 nm across wavelength range Wavelength repeatability 0 2 nm Photometric range 0 to 4 000 OD Photometric resolution 0 001 OD Photometric accuracy linearity plate 0 2 0 OD...

Страница 37: ...urement conditions endpoint column priority for dual wavelength measurements calibrate off 9 seconds single wavelength 19 seconds dual wavelength 425 650 nm 29 seconds single wavelength 59 seconds dua...

Страница 38: ...n Read chamber Isothermal when temperature regulation is not enabled Range 4 C above ambient to 45 C when temperature regulation enabled The ambient temperature must be 20 C to achieve temperature reg...

Страница 39: ...4 mm typical for 3 mL volume cells Test tubes 12 x 75 mm test tubes with test tube cover read in the cuvette chamber Compatibility SpectraMax Plus 384 Item Description Display 2 x 20 character back l...

Страница 40: ...croplate Readers User Guide 40 0112 0126 B Item Description Size h x w x d 220 mm 8 6 x 580 mm 22 8 x 380 mm 15 Weight 13 6 kg 30 lb Power consumption 250 W Line voltage and frequency 90 250 VAC autor...

Страница 41: ...tric range 0 to 4 000 OD Photometric resolution 0 001 OD Photometric accuracy linearity plate 0 2 0 OD 340 850 nm 1 0 and 0 006 OD Photometric precision repeatability 0 2 0 OD 340 850 nm 1 0 and 0 003...

Страница 42: ...4 Item 96 wells 384 wells Endpoint standard read 9 seconds single wavelength 19 seconds dual wavelength 425 650 nm 29 seconds single wavelength 59 seconds dual wavelength 425 650 nm Kinetic read inter...

Страница 43: ...Temperature regulation diagnostics Temperature regulation system is continuously monitored and updated Evaporation Plate lid required to minimize evaporative cooling Recommended plate Flat bottom pla...

Страница 44: ...lel 25 pin to Centronics double shielding required Supported plates All 96 well and strip well plates including lids General Instrument SpectraMax 340PC384 Item Description Operating temperature 5 C t...

Страница 45: ...OD 190 850 nm 1 0 and 0 003 OD Stray light 0 05 at 340 nm Photometric stabilization Instantaneous Photometric drift None continuous referencing of monochromatic input Calibration Automatic before eve...

Страница 46: ...escription Read chamber Isothermal when temperature regulation is not enabled Range 4 C above ambient to 45 C when temperature regulation enabled The ambient temperature must be 20 C to achieve temper...

Страница 47: ...trol panel 8 key membrane keypad Self diagnosis Continuous on board diagnostics Spill control Drawer mechanism and read chamber assembly protected from accidental spillage by drainage ports Computer i...

Страница 48: ...Microplate Readers User Guide 48 0112 0126 B Item Description Size h x w x d 220 mm 8 6 x 580 mm 22 8 x 380 mm 15 Weight 13 6 kg 30 lb Power consumption 250 W Line voltage and frequency 90 250 VAC aut...

Страница 49: ...1 0 and 0 006 OD Photometric precision repeatability 0 2 0 OD 340 850 nm 1 0 and 0 003 OD Stray light 0 05 at 340 nm Photometric stabilization Instantaneous Photometric drift None continuous referenci...

Страница 50: ...Resolution 0 1 C Accuracy 1 0 C for plate chamber Temperature uniformity at equilibrium 0 5 C at 37 C Chamber warm up time 15 30 minutes measured on air after initiation of temperature regulation Tem...

Страница 51: ...el 25 pin to Centronics double shielding required Supported plates All 96 well and strip well plates including lids General Instrument VersaMax Item Description Operating temperature 5 C to 40 C Opera...

Страница 52: ...la norme NMB 001 du Canada ISM Equipment Classification Group 1 Class A This equipment is designated as scientific equipment for laboratory use that intentionally generate and or use conductively coup...

Страница 53: ...5 cm Their fill volumes differ only because of their different internal width and chamber height dimensions Standard and Semi micro Cuvettes Several brands available Internal Width Minimum Volume Maxi...

Страница 54: ...Standard Semi Micro Micro Hellma Cat No 100 104 105 004 104 002 108 002 105 Internal Dimensions 10 x 10 4 x 10 4 x 10 2 x 10 2 x 10 2 x 10 Fill Volume 4 mL 1 4 mL 600 L 700 L 500 L 300 L Ultra Micro C...

Страница 55: ...y of Molecular Devices LLC or their respective owners Specifications subject to change without notice Patents www moleculardevices com productpatents FOR RESEARCH USE ONLY NOT FOR USE IN DIAGNOSTIC PR...

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