Chapter 1: Introduction
0112-0102 E
11
One plate drawer adapter is provided with the instrument. The plate adapter is required
for optimum performance with standard 96-well and 384-well format plates for all top-
read applications.
Note:
Although not recommended, you can also use strip well plates in the
instrument. When you use strip-wells, use high quality support frames or holders. To
avoid warping, you should fill the frame or holder with empty strips.
Supported Cuvettes
For a cuvette read, you must ensure that the meniscus is above the light beam in a standard
cuvette and that the sample chamber is aligned properly with the beam in a microcuvette.
The light beam is 0.625 in (15.87 mm) above the cuvette bottom.
Use the cuvette port for Absorbance, Fluorescence, and Luminescence (secondary
mode) read modes.
To allow transmission of the deep UV spectra when you do a read at wavelengths below
340 nm, you must use special UV-transparent cuvettes or quartz cuvettes.
Endpoint, Kinetic, and Spectrum read types are available for the Absorbance and
Fluorescence read modes with cuvettes.
Note:
You can use a 12 x 75 mm test tube with a test tube cover. You can order the
test tube cover from Molecular Devices. See
.
Shake
The software enables you to define settings to mix of the contents of the wells by shaking the
plate linearly along the long axis at preset intervals.
For the Endpoint read type, you can shake the plate for 0 to 999 seconds before the read
at each wavelength you set.
For the Kinetic read type, you can shake the plate for 0 to 999 seconds before the initial
read, and/or before each subsequent read.
Use of shake is recommended for ELISAs and other solid-phase, enzyme-mediated reactions
to enhance accuracy.
Temperature Regulation
The temperature in the plate chamber and cuvette chamber is isothermal, both at ambient
and when you turn on the incubator. When you turn on the incubator, you can set the
temperature from 4°C above ambient to 45°C. The ambient temperature must be at least
20°C to reach the 45°C operating temperature. The operating humidity must be non-
condensing, and can range from 0 to 70%.
