Molecular Devices FLIPR Tetra, Руководство пользователя

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Running an Experiment
180 0112-0109 H
Dye Loading Using the FLIPR Membrane Potential
Assay Kit
Preparation of Loading Buffer
The following procedure is designed for preparation of the Loading
Buffer for either of the Membrane Potential Assay Kits, BLUE (R8034) or
RED (R8123) in the Bulk format.
Blue or Red
To prepare loading buffer:
1. To prepare a 1X Reagent Buffer dilute 10 mL of 10X Reagent
Buffer (Component B) to 100 mL with distilled water. Adjust to
pH 7.4 with
1 N NaOH.
Depending upon the cell type and application, the Assay Buffer
as provided with the FLIPR
®
Membrane Potential Assay Kit may
not be the ideal choice. If so, alternative buffers may be used at
your discretion in order to achieve optimal results.
2. Remove a vial of FLIPR
®
Membrane Potential Assay Reagent
(Component A).
3. Dissolve contents of vial completely by adding 100 mL of 1X (96
and 384) or
67 mL of 3X (1536) Assay Buffer. Mix by repeated pipetting until
the contents are completely dissolved.
Note: Occasionally, a white precipitate forms in the 10X
Reagent Buffer bottle. This is normal and will not affect the
assay.
Note: For best results, dissolve the contents of the vial with 10
mL of 1x Assay Buffer, and then perform several washes of the
vial using 1x Assay Buffer to yield a total volume of 100 mL (96
or 384) or 67 mL (1536).
Note: Components supplied are sufficient for proper cell
loading. For optimum results, it is important NOT to add any
additional reagents or change volumes and concentrations.