Molecular Devices FLIPR Tetra, Руководство пользователя

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System Overview
14 0112-0109 H
Light-emitting diodes (LEDs) in the FLIPR
®
Tetra System produce light
at distinct wavelength ranges to excite the fluorescent dye that has
been added to the cells in the read plate wells. The entire well plate
bottom is illuminated. Fluorescent light emitted by the dye—again, for
the entire plate—passes through an emission filter before being
captured in a CCD camera, standard EMCCD or Aequorin ICCD.
Fluorescence is measured from each well independently, and converted
into a numerical value. The FLIPR
®
Tetra System can be configured with
two LED banks and three emission filters, allowing the software
configuration of up to four excitation/emission wavelength
combinations (‘read modes’). Thus, up to four different fluorescence
effects can be measured within a single experiment.
Luminescence Mode
The FLIPR
®
Tetra System also provides luminescence mode with the
Aequorin, ICCD camera option. The instrument has a light-tight
enclosure so that it can operate in luminescence mode and a
specialized high-sensitivity ICCD camera can be installed in place of the
standard EMCCD camera. The ICCD camera is mounted directly
beneath the read plate, so images are taken of the entire bottom of the
plate. For cell suspension experiments, an integrated Cell Reservoir
allows uniform cell suspension to be pipetted directly into the read
plate. From the 3 filter positions available on FLIPR
®
Tetra System, it is
recommended to have one open position, so no filter will be used
during luminescence assay.
As with fluorescence, luminescence is measured from each well
independently, and converted into a numerical value.
System Requirements
This section provides a brief overview of electrical, physical and
environmental requirements of the FLIPR
®
Tetra System. Please refer
to the FLIPR
®
Tetra System Pre-Installation Guide for full details.
Electrical
FLIPR
®
Tetra System consumes 5 A continuous and 9 A peak of 110 V
power and requires 90–240 VAC power source at 50–60 Hz which
equates to 2.5 A continuous and up to 4.5 A peak at 240 VAC/50 Hz.
The system is supplied with a power cord appropriate for the country it
was shipped to. Additional shared outlets are required for computer and
monitor. A power strip is acceptable for providing the additional outlets
for the computer and monitor.