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07.
support@minipcr.com
bluegel
TM
user’s guide
DIRECTIONS F
OR USE
DIRECTIONS FOR USE
CASTING A GEL
Place the gel tray inside the casting platform. Place on a level surface to ensure uniform
gel thickness.
Determine the percentage gel to make:
Place flask in microwave (~30 seconds) or on a hot plate until all the agarose is dissolved.
The agarose/buffer mix is ready when no agarose particles are visible upon swirling.
CAUTION: liquid may bubble over the mouth of the flask and cause burns. Handle with
care using protective equipment.
Let agarose/buffer mix cool for ~2-3 minutes and add 2
μ
l of GelGreen
TM
DNA stain
10,000X stock (1
μ
l per 10 ml of TBE). Swirl well to mix. See Appendix B for additional
DNA staining dyes that work with blueGel
TM
.
Weigh the desired amount of agarose according to the chart above and add it to a 100
ml size flask(or larger) containing 20 ml of 1X TBE electrophoresis buffer. Mix well by
swirling.
Tip: If pouring more than one gel, agarose and buffer quantities can be multiplied by
the number of gels to be poured. Increase heating time by ~15 sec per additional gel
and use a larger flask.
Note: if using two rows of wells (two combs) resolution will be reduced due to shorter
separation distance.
* Warning: blueGel
TM
is designed to work best with 0.5 to 1.0X TBE (Tris Borate EDTA)
buffer. Use of other buffers such as TAE or SB may result in impaired performance.
1 --
4 --
5 --
3 --
2 --
Size of DNA
to separate
Gel percentage
(%)
Agarose
(g)
1x TBE*
(ml)
600 bp to 12 kb
0.8
0.16
20
500 bp to 10 kb
1
0.2
20
400 bp to 7 kb
1.5
0.3
20
200 bp to 5 kb
2
0.4
20
60 bp to 2 kb
3
0.6
20