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Product Manual

PDQeX Nucleic Acid Extractor

prep

GEM

forensic

GEM

phyto

GEM

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Содержание PDQeX

Страница 1: ...Product Manual PDQeX Nucleic Acid Extractor prepGEM forensicGEM phytoGEM ...

Страница 2: ...rview Page 4 Contact Page 16 Device Set up Page 5 Loading and Unloading Pages 6 8 Settings Page 9 Date and Time Page 10 UV Treatment Pages 10 11 Pre defined Programs Page 12 Custom Programs Page 13 Error Messages Page 14 Cleaning Sterilization and Maintenance Page 15 ...

Страница 3: ...Hz 96 watts at peak draw Pre installed and user programmable 34 120 C 22 x 18 x 18 5 cm L x W x H 8 5 x 7 x 7 25 4 8 kg 10 6 lbs PDQeX Extraction Reagents consist of a cocktail of thermophilicproteinaseandmesophiliccellwalldegrading enzymes that systematically lyse cells destroy nucleases digest proteins and release nucleic acids Each reagent kit is specially formulated for specific sample types P...

Страница 4: ...xtractor cartridge forces the extract through a special column The columns are tailored to each sample type and designed to remove anything that may inhibit Taq DNA polymerase or enzymes used in most other downstream processes 4 The samples are ejected into PCR tubes in a standard format 8 strips or 3x8 strips Step 2 Cell lysis DNA extraction and purification Sample preparation prior to extraction...

Страница 5: ...ocket Back of the PDQeX Front of the PDQeX Cartridge lifting tool Hinged flap to secure cartridges Drawer for collection tube rack Collection tube rack Do not place the PDQeX close to a RF transmitter A low power transmitter such as a cordless or mobile phone should be at least 2M from the PDQeX A powerful transmitter such as a commercial TV or radio broadcast antenna should be at least 25M away T...

Страница 6: ... ensure the lifting tool is in place making it easier to remove spent cartridges after the run is complete Pull out the collection tube rack and place 0 2 ml PCR tubes firmly into the rack The rack will accommodate up to three 8 strip PCR tubes Remember where you have placed the collectiontubes Youwillneedtoplacethe cartridges in the same position in the cartridge block Replace the rack into its s...

Страница 7: ... cartridges are loaded lower the hinged flap onto the caps of the cartridges and close the sliding door Make sure the cartridge positions in the cartridge tray correspond to the collection tubes below otherwise you will contaminate the device WARNING The lower end of the PDQeX cartridge is designed to fit inside the collector tube If it is above the upper lip of the tube there is a risk of cross c...

Страница 8: ...r cartridges and dispose Slide out the drawer Remove and cap the collection tubes and store at 20 C The door of the device will lock while the block is hot For sequential runs make sure the block temperature has fallen below the first temperature used in your program Carefully remove tubes to prevent contamination of the block Tubes cannot be re used if you abort a run Notes ...

Страница 9: ...e information 3 Reset to factory defaults Future releases will allow you to pick your preferred language Running your program ThePDQeXiscontrolledbyatouch screen Thefollowingsectionprovidesanoverviewtohelpnavigatethrough the controls 1 Home Screen 2 System Settings 2 1 Factory Reset The device arrives with a number of pre installed programs A factory reset restores the programs if lost However ify...

Страница 10: ... situated below the collection tube drawer The drawer should be removed before treatment to allow the light to access the lower side of the heating block The other light is above the heating block During UV treatment the sliding door will be locked WARNING DonotoperatetheUVlightwithoutclosingthedoor Avoid lookingdirectly at the UV light The UV treatment will only remove superficial contamination F...

Страница 11: ...nt on the home screen The procedure takes 30 minutes and can be interrupted at any time Press Interrupt to stop the UV treatment After interrupting the program press Return to go back to the home screen After the treatment is complete press Return to go back to the home screen ...

Страница 12: ...Extract Select the program you want to run The temperature regimen will be shown Press Start The PDQeX will switch to a display showing the progress of the program At any time the program can be terminated but the samples and tubes cannot be reused Once the run is complete a prompt will appear to return to the extraction screen ...

Страница 13: ...on the dashed line at the top of the screen and give the custom program a name A program cannot be saved without a name Press on the temperature time symbols to add temperature and time values 5 Writing a Custom Program on the PDQeX Use the number keypad to enter temperature C and time minutes values PressSave Youmustaddafinalcoolstep before the program can be saved When you have completed all the...

Страница 14: ...he drawer is not in place or is crooked the device will not run This prevents DNA from being contaminated Make sure the sliding door is fully closed before running the PDQeX The block is hot and could burn fingers This message is a reminder that the same tubescannotberuntwiceifarunhasbeen aborted A few common mistakes may be detected by the device ...

Страница 15: ...d wipes Aluminum parts of this device should not be soaked in virkon or bleach Extended use of chemical agents such as these will corrode the parts The following label may be displayed on the device The PDQeX has been designed to require no operator maintenance Cleaning and Sterilization WARNING Do not get liquids inside the device If bleach must be used be sure ALL of it is removed Safety Label H...

Страница 16: ...perty of their respective owners forensicGEM phytoGEM prepGEM RNAGEM and MicroGEM are trademarks in use by the MicroGEM group Registered with the United States Patent and Trademark Office MicroGEM is committed to minimal packaging and a vibrant sustainable world For information regarding device compliance with various agency standards contact info microgembio com ...

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