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MicroCal iTC
200
Washing Module User Guide
19
Part 5: Washing Module Basic Operation
Preparation
Before running an experiment, you may need to perform one or all of the following tasks:
Bottle Filling
Use this procedure to fill the bottles in preparation for using the washing module. Although the
bottles do not have to be full before you begin a procedure, you should ensure that there is
sufficient volume in each bottle to perform the required procedure. There are three bottles
that you must maintain:
Water: bottle with blue top
Methanol: bottle with red top
Buffer: bottle with white top
Procedure:
1. Verify that the system is in an idle state.
2. Unscrew the plastic cap of the bottle by turning it
counter-clockwise.
3. Fill bottle using standard lab procedures.
4. After filling, tighten the cap by turning clockwise
until snug.
Priming Bottles
Use this procedure to prime the tubing from the bottles to the washing module to ensure that
the full volume is delivered. This procedure is required only if the tubes leading from the bottles
to the washing module have been drained of fluid and contain air.
1. Make sure all bottles have sufficient volume and all fluid lines are connected.
2. On the Instrument Controls tab, click Cell Buffer Rinse (Short).
3. On the Instrument Controls tab, click Syringe W ash (Long).
After lines are primed (visibly clear of air), the system uses the majority of the remaining
procedure time to dry the syringe. This occurs with approximately eight minutes remaining in
the procedure. You can let the procedure finish or click Cancel at this time.
MicroCal iTC200 Washing Module MAN0582
Содержание MicroCal iTC200
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