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MACHEREY-NAGEL – 08 / 2013, Rev. 01

20

6.4  Product  use  restriction / warranty

NucleoSpin

®

 Plasmid EasyPure kit components are intended, developed, designed, 

and sold FOR RESEARCH PURPOSES ONLY, except, however, any other function of 

the product being expressly described in original MACHEREY-NAGEL product leaflets.

MACHEREY-NAGEL products are intended for GENERAL LABORATORY USE 

ONLY! MACHEREY-NAGEL products are suited for QUALIFIED PERSONNEL ONLY! 

MACHEREY-NAGEL products shall in any event only be used wearing adequate 

PROTECTIVE CLOTHING. For detailed information please refer to the respective 

Material Safety Data Sheet of the product! MACHEREY-NAGEL products shall 

exclusively be used in an ADEQUATE TEST ENVIRONMENT. MACHEREY-NAGEL 

does not assume any responsibility for damages due to improper application of our 

products in other fields of application. Application on the human body is STRICTLY 

FORBIDDEN. The respective user is liable for any and all damages resulting from such 

application.

DNA/RNA/PROTEIN  purification  products  of  MACHEREY-NAGEL  are  suitable  for 

IN VITRO

-USES ONLY!

ONLY MACHEREY-NAGEL products specially labeled as IVD are also suitable 

for 

IN VITRO

-diagnostic use. Please pay attention to the package of the product. 

IN VITRO

-diagnostic products are expressly marked as IVD on the packaging.

IF THERE IS NO IVD SIGN, THE PRODUCT SHALL NOT BE SUITABLE FOR 

IN VITRO

-DIAGNOSTIC USE!

ALL OTHER PRODUCTS NOT LABELED AS IVD ARE NOT SUITED FOR ANY 

CLINICAL USE (INCLUDING, BUT NOT LIMITED TO DIAGNOSTIC, THERAPEUTIC 

AND/OR PROGNOSTIC USE).

No claim or representations is intended for its use to identify any specific organism 

or for clinical use (included, but not limited to diagnostic, prognostic, therapeutic, or 

blood banking). It is rather in the responsibility of the user or - in any case of resale of 

the products - in the responsibility of the reseller to inspect and assure the use of the 

DNA/RNA/protein purification products of MACHEREY-NAGEL for a well-defined and 

specific application. 
MACHEREY-NAGEL shall only be responsible for the product specifications and the 

performance range of MN products according to the specifications of in-house quality 

control, product documentation and marketing material.

This MACHEREY-NAGEL product is shipped with documentation stating specifications 

and other technical information. MACHEREY-NAGEL warrants to meet the stated 

specifications. MACHEREY-NAGEL´s sole obligation and the customer´s sole remedy 

is limited to replacement of products free of charge in the event products fail to perform 

as warranted. Supplementary reference is made to the general business terms and 

conditions of MACHEREY-NAGEL, which are printed on the price list. Please contact 

us if you wish to get an extra copy. 
There is no warranty for and MACHEREY-NAGEL is not liable for damages or defects 

arising in shipping and handling (transport insurance for customers excluded), or 

out of accident or improper or abnormal use of this product; defects in products or 

Plasmid DNA purification

Содержание NucleoSpin 740727.10

Страница 1: ...Plasmid DNA purification User manual NucleoSpin Plasmid EasyPure August 2013 Rev 01...

Страница 2: ...ww mn net com NucleoSpin Plasmid EasyPure 1 Cultivate and harvest bacterial cells 12 000 x g 30 s 2 Cell lysis 150 L Buffer A1 250 L Buffer A2 RT up to 2 min 350 L Buffer A3 3 Clarification of the lys...

Страница 3: ...ion 6 2 1 Basic principle 6 2 2 Kit specifications 6 2 3 Growth of bacterial cultures 7 2 4 Elution procedures 8 3 Storage conditions and preparation of working solutions 10 4 Safety instructions 11 4...

Страница 4: ...Buffer A2 5 mL 15 mL 75 mL Neutralization Buffer A3 5 mL 20 mL 100 mL Wash Buffer AQ Concentrate 6 mL 6 mL 30 mL Elution Buffer AE 15 mL 15 mL 30 mL Liquid RNase A 2 mg 6 mg 30 mg NucleoSpin Plasmid...

Страница 5: ...e detailed protocol sections of this user manual if the NucleoSpin Plasmid EasyPure kit is used for the first time Experienced users however may refer to the Protocol at a glance instead The Protocol...

Страница 6: ...g step with Buffer AQ Pure plasmid DNA is finally eluted under low ionic strength conditions with slightly alkaline Buffer AE 5 mM Tris HCl pH 8 5 2 2 Kit specifications The NucleoSpin Plasmid EasyPur...

Страница 7: ...roth or CircleGrow can be used In this case bacteria grow faster reach the stationary phase much earlier than in LB medium 12 h and higher cell masses can be reached However this does not necessarily...

Страница 8: ...Recommended culture volumes for NucleoSpin Plasmid EasyPure OD600 1 2 3 4 5 6 Culture volume 15 mL 8 mL 5 mL 4 mL 3 mL 2 mL Note if too much bacterial material is used the lysis and precipitation step...

Страница 9: ...ifuge Apply a second aliquot of elution buffer incubate and centrifuge again Thus about 85 100 of bound nucleic acids are eluted with the standard elution volume at a high concentration Elution Buffer...

Страница 10: ...he preparation Storage of Buffer A2 below 20 C may cause precipitation of SDS If salt precipitate is observed incubate buffer at 30 40 C for several minutes and mix well until all precipitate is redis...

Страница 11: ...skin Ber hrung mit der Haut vermeiden S 26 In case of contact with eyes rinse immediately with plenty of water and seek medical advice Bei Ber hrung mit den Augen gr ndlich mit Wasser absp len und Arz...

Страница 12: ...siv sein H 302 Harmful if swallowed Gesundheitssch dlich bei Verschlucken H 315 Causes skin irritation Verursacht Hautreizungen H 317 May cause an allergic skin reaction Kann allergische Hautreaktione...

Страница 13: ...n irritation or a rash occurs Get medical advice attention Bei Hautreizung oder ausschlag rztlichen Rat einholen rztliche Hilfe hinzuziehen P 337 313 Get medical advice attention Bei anhaltender Hautr...

Страница 14: ...A2 Attention Check Buffer A2 for precipitated SDS If a white precipitate is visible warm the buffer for several minutes at 30 40 C until precipitate is dissolved completely Cool buffer down to room t...

Страница 15: ...w through and make sure the spin cup outlet does not touch the wash buffer surface Otherwise repeat the centrifugation step Note To reduce ethanol carry over to a minimum for better performance in dow...

Страница 16: ...oo many bacterial cells used See table 3 for maximum amount of cells Poor plasmid yield Incomplete lysis of bacterial cells See Possible cause and suggestions above No or insufficient amounts of antib...

Страница 17: ...strains used Especially when working with nuclease rich strains keep plasmid preparations on ice or frozen in order to avoid DNA degradation Inappropriate storage of plasmid DNA Quantitate DNA directl...

Страница 18: ...through and repeat centrifugation Elution of plasmid DNA with TE buffer EDTA may inhibit sequencing reactions Repurify plasmid DNA and elute with Buffer AE or water Alternatively the eluted plasmid DN...

Страница 19: ...Buffer A3 740913 1 1 L Buffer AQ Concentrate for 100 mL Buffer AQ 740995 20 mL Buffer AE 740917 1 1 L Liquid RNase A 740397 250 mg Collection Tubes 2 mL 740600 1000 6 3 References Birnboim H C and J D...

Страница 20: ...PRODUCTS NOT LABELED AS IVD ARE NOT SUITED FOR ANY CLINICAL USE INCLUDING BUT NOT LIMITED TO DIAGNOSTIC THERAPEUTIC AND OR PROGNOSTIC USE No claim or representations is intended for its use to identi...

Страница 21: ...xpressed or implied The warranty provided herein and the data specifications and descriptions of this MACHEREY NAGEL product appearing in MACHEREY NAGEL published catalogues and product literature are...

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