6. Routine Maintenance and Trouble Shooting
No routine maintenance is required. There are no user-replaceable components or sub-assemblies in the
CELESTA light engine. Opening the light engine enclosure will void the manufacturer’s warranty. In the event
that the light engine fails to perform in accordance with the specifications listed on the certificate of
conformance, please contact Lumencor Technical Support for assistance, as directed in Section 7.
7
. Customer Support
For technical support of the CELESTA light engine, please contact Lumencor by phone at 503-213-4269 or
through e-, mail at
Please be prepared to provide the 4- or 5-digit serial
number of the light engine. Any light engine returned to Lumencor for repairs or upgrades requires a pre-
issued return material authorization (RMA) number. To obtain a RMA number, submit the online request form
at
http://lumencor.com/support/lumencor-rma-request-form
. It is the customer’s responsibility to properly
Troubleshooting Procedures
Problem
Check the following
No response to serial (USB or
RS-232) commands
Check that the LEGACY or STANDARD command mode selection
(GUI Settings; Figure 5) is compatible with the software driver.
No response to TTL trigger
commands
Check that
TTL inputs
are ENABLED (GUI Settings; Figure 5) and
that the
TTL polarity
setting is consistent with the trigger inputs.
Also check that all serial ON/OFF controls are in the OFF state.
No light output in response to
source
ON
command (serial or
TTL).
There are 4 interlocks that need to be closed, check all of these:
1. Control key must be inserted in front panel and turned to “on”
position¶.
2. Liquid light guide must be inserted in light output receptacle¶.
3. Remote interlock jumper must be inserted in rear panel¶.
4. External gate jumper must be inserted in front panel (Figure 3).
Unusually weak fluorescence
signals across all detection
channels
Weak fluorescence in all detection channels (DAPI, FITC, TRITC,
Cy5 etc) is likely to be due to poor light transmission by the liquid
light guide, the collimating adaptor or another distal component
of the microscope optical path and not to abnormally low light
output from the light engine.
Unusually weak fluorescence
signals in a single detection
channel (e.g. DAPI)
1. Check that the light engine power output reading in the Web
GUI (Figure 4) is normal.
2. Check that the dichroic beamsplitter and emission bandpass
filter in the microscope are compatible with light engine
excitation filter specifications shown on the certificate of
conformance (Figure 1).
High, spatially uniform
fluorescence background
Check that the dichroic beamsplitter and emission bandpass filter
in the microscope are compatible with light engine excitation filter
specifications shown on the certificate of conformance (Figure 1).
¶ Open interlocks for these conditions will be indicated by a red status indicator in the lower right
corner of the GUI control screen (Figure 4)
CELESTA Light Engine
®
Instruction Manual
15