3
6. Close doors and relatch by pressing down on the
white latches so that the assembly looks like that
shown in figure 5.
7. Place stirring bar, if desired, into bottom of reservoir
in stirring corral.
3.2
Running the Gel
1. Place core assembly into lower reservoir. The anode
(red) and cathode (black) electrodes are color-coded
on both the core/cassette assembly and lower
reservoir. Ensure the red dot on the cassette
assembly is on the same side as the red receptacle
on the lower reservoir. Fill core upper reservoir with
freshly prepared buffer (~ 190 ml).
NOTE: If any buffer is spilled into banana jack
receptacle in lower reservoir, dry completely
using compressed air! Failure to do this will
result in accelerated banana jack corrosion.
Banana jack
receptacles
Figure 6
Figure 4
2. See section 5.3 for recommended buffers.
Pour only enough freshly prepared buffer into
lower chamber so that the final buffer level is
just below bottom of sample wells. Using a
pipette or syringe, thoroughly flush out the
wells.
3. Load samples. If outer lanes do not contain
sample, it is recommended that you run
standards and/or fill outer lanes with loading
buffer to reduce smiling and wrap-around
effects.
4. Attach safety cover and turn on magnetic stirrer
(if desired).
Figure 5
Figure 7
5. Connect the leads to the power supply, matching the
color-coded red-to-red and black-to-black.
See
Section 5.1 for recommended power conditions.
Begin separation by electrophoresis. Figure 7
shows the closed unit ready for electrophoresis.
