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BioResearch

Amaxa™ HT Nucleofector™ hardware manual

For research use only

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Содержание Amaxa HT Nucleofector

Страница 1: ...BioResearch Amaxa HT Nucleofector hardware manual For research use only...

Страница 2: ...e non transferable right to use the system as well as Lonza s proprietary Nucleofector technology only for research conducted by the buyer whether the buyer is an academic or for profit entity For fur...

Страница 3: ...em components 8 2 6 System set up instructions 10 2 7 Instructions for using the HT Nucleofector system 11 3 Running an experiment 12 3 1 Processing a 384 well Nucleocuvette plate 12 3 2 System shutdo...

Страница 4: ...provides cell type specificoptimizedprotocolsformanydifferentcelllinesandprimarycells Transfection of any cell With over 160 protocols for cell lines and primary cells optimized by Lonza sR Dteamandm...

Страница 5: ...eofector system meets your screening requirements Experience the benefits of the HT Nucleofector system Automation compatible full integration into liquid handling platforms Fast process 384 samples i...

Страница 6: ...esearch conducted by the buyer whetherthebuyerisanacademicorfor profitentity Forfurtherdetails about the license refer to chapter 5 For license information contact Lonza Cologne GmbH Phone 49 221 9919...

Страница 7: ...eonprecautionsto be taken before further use Donotopenthedevicehousing Undernocircumstancesshouldcircuit components be interfered with as they can deliver an electric shock even when the system is not...

Страница 8: ...fector solutions in a biohazard container Refer to your local waste management organization and to the relevant laboratory safety instructions for proper disposal practices 2 5 HT Nucleofector system...

Страница 9: ...upply 7 8 10 9 12 11 HT Nucleofector plate handler The HT Nucleofector plate handler serves as the contacting unit of the system Transfection of the samples occurs in 384 well Nucleocuvette plates tha...

Страница 10: ...technical specialist Follow the instructions below if the instrument is moved from its original location ConnecttheHVportoftheHTNucleofector powersupply 10 tothe HVportontheplatehandlerUnit 13 usingth...

Страница 11: ...m execution optional Software manual 10 Remove 384 well Nucleocuvette plate from the plate handler and transfer samples to appropriate culture plates Optimized protocol 11 Check result file on the com...

Страница 12: ...re that plate position A01 is in the upper left corner Place the plate gently into the plate handler retainer position 1 Afterloadingthe384 wellNucleocuvette plate starttheexperimentfrom the computer...

Страница 13: ...this may negatively influence the viability of the cells after Nucleofection For detailed recommendations on passage number cell density and confluency refer to the relevant optimized protocol Low tr...

Страница 14: ...tch of the power supply 2 Disconnect the main power cable from the power supply power inlet 3 Remove the small plastic cap on top of the plate handler housing see Figure 4 1 to access the emergency li...

Страница 15: ...esentations and warranties Lonza Cologne represents and warrants that it is not aware of any legal deficiencies of the patents and that to its knowledge it has the right to dispose of the patents Lonz...

Страница 16: ...50 45 cm 5 3 19 6 17 7 in Manufacturing date The year of manufacture is indicated by the second and third digit of the serial number e g a device with the serial number x11xxxxx was manufactured in 20...

Страница 17: ...17 Notes 6...

Страница 18: ...18 BioResearch HT Nucleofector hardware manual Notes...

Страница 19: ...19 Notes...

Страница 20: ...ed Use in any other application requires a license from Evrogen To obtain such a license please contact Evrogen at license evrogen com The information contained herein is believed to be correct and co...

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