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Leica VT1200 / Leica VT1200 S

Vibrating-blade microtome

Instructions for Use

English

Order No.: 14 0481 80101 - Revision L

Always keep this manual with the instrument.

Read carefully before working with the instrument.

Version 1.8, Revision L - 08.2022

Содержание VT1200

Страница 1: ...1200 S Vibrating blade microtome Instructions for Use English Order No 14 0481 80101 Revision L Always keep this manual with the instrument Read carefully before working with the instrument Version 1...

Страница 2: ......

Страница 3: ...information regarding the contents or technical details of the present Instructions for Use are not to be considered warranted characteristics of our products These are determined only by the contrac...

Страница 4: ...Description of the typical application 24 5 2 Control elements on the Leica VT1200 control panel 25 5 2 1 Moving the specimen receptacle vertically 25 5 2 2 Fine adjusting the blade to specimen proxim...

Страница 5: ...cimens 53 9 1 1 Buffer trays 53 9 1 2 Specimen plate 54 9 1 3 VibroCheck 55 9 1 4 Blades 56 9 1 5 Microscope assembly 56 9 1 6 Magnifier assembly 56 9 1 7 Installing the magnifier support or microscop...

Страница 6: ...ations that have to be displayed on the input screen are displayed as bold gray text Symbol Title of the symbol Function key Save Description Fuction keys to be pressed on the instrument are displayed...

Страница 7: ...The Regulatory Compliance Mark RCM indicates a device s compliance with applicable ACMA technical standards of New Zealand and Australia that is for telecommunications radio communications EMC and EME...

Страница 8: ...sonnel designated to operate this instrument must read these Instructions for Use carefully and must be familiar with all technical features of the instrument before attempting to operate it 1 4 Inten...

Страница 9: ...devices In order to maintain this condition and to ensure safe operation the user must follow the instructions and warnings contained in this operating manual The current EC Declarations of Conformit...

Страница 10: ...he power plug before replacing the fuse The use of fuses other than those installed at the factory is not permitted 2 3 Transport unpacking and setting up When unpacking the instrument compare the par...

Страница 11: ...tude meters up to 2000 m above sea level Temperature operation min max min 10 C to max 35 C Relative humidity operation min max max 60 Temperature transit min max 29 C to 50 C Temperature storage min...

Страница 12: ...ness selection manual in 1 m increments 3 1 1 Technical data for the Leica VT1200 S Electrical Specifications Nominal supply voltage 100 V 240 V Nominal supply frequencies 50 60 Hz Mains supply voltag...

Страница 13: ...ctioning frequency 10 85 Hz 10 Amplitude from 0 3 mm in 0 05 mm increments Cutting range 45 mm Sectioning window 0 5 mm 45 mm Specimen orientation rotating 360 Specimen plate swiveling 0 10 Electrical...

Страница 14: ...14 Version 1 8 Revision L Instrument characteristics 3 3 2 General overview Leica VT1200 Leica VT1200 S Fig 1 7 6 4 5 2 3 1 8 9 10...

Страница 15: ...e for specimens 1 cm in height 4 Magnifier 15 Specimen plate for specimens 2 cm in height 5 Microscope 16 Specimen plate directional 6 Module LED Hi Power spots 2 arm 17 Cyanoacrylate adhesive 7 Modul...

Страница 16: ...e 1 m 10 m or 100 m steps 5 Quickly moves the specimen upwards press the button until the desired position is reached 15 Pressing this button toggles between m and m actively illuminated 6 Quickly mov...

Страница 17: ...when the window edge is set 2 Setting dial 1 for blade feed rate SPEED AMPLitude or section thickness for automatic mode AUTO FEED 8 Second cutting window edge LED illuminates when the window edge is...

Страница 18: ...n CAUTION Keep it pressed down until the desired position is reached When the end position is reached the LED lights up 16 Sets the totalized section thickness display m to 0 Note The Leica VT1200 S i...

Страница 19: ...10 Buffer tray metal assembly 14 0481 42084 If the supplied local power cord is defective or lost please contact your local Leica Biosystems representative Note When ordering additional accessories co...

Страница 20: ...r order immediately 4 2 Packing and setting up the instrument Note Ensure that the instrument is standing on a work surface that is as free of vibrations as possible Before each transport the handles...

Страница 21: ...21 Leica VT1200 Leica VT1200 S Installation 4 Fig 6 3 4 Fig 7 5 6 3 Insert the accessories box Fig 7 6 contains accessories and fill it with packing material Fig 8 7...

Страница 22: ...w it into place using 8 Phillips screws Fig 8 9 4 3 Before commissioning the instrument Fig 9 1 1 Once the instrument is in its final location unscrew the transport handles Fig 9 1 from the instrument...

Страница 23: ...to the socket Fig 10 4 4 Install the ice tray Fig 10 5 5 Install the buffer tray Fig 10 6 6 Install any optional accessories such as the magnifier microscope foot switch etc according to p 53 9 1 Ext...

Страница 24: ...fer tray can be installed safely 3 Insert the buffer tray into the ice tray and cover it with the lid Fill the ice tray with crushed ice 4 Remove the cover and fill the buffer tray with pre cooled phy...

Страница 25: ...cle is moving downwards the specimen receptacle comes to a stop The current position appears on the m display lowest position 0 The m display does not change while the specimen receptacle is in motion...

Страница 26: ...s When each end point has been reached the corresponding LED lights up in the key forward back 5 2 4 Selecting the sectioning parameters SPEED Blade feed rate upper display LED mm s The desired blade...

Страница 27: ...witched back on Blade feed rate SPEED Blade feed rate SPEED Selected amplitude AMPL Selected amplitude AMPL Saved section thickness AUTO FEED The LED illumination is switched on automatically You can...

Страница 28: ...Toggling from MAN to AUTO An audible warning signal sounds when these keys are pressed The following functions keys become enabled again in automatic mode Cutting window edges that have already been s...

Страница 29: ...ng the setting dial counterclockwise lowers the specimen minus sign ditto The step size 1 10 or 100 m can be selected by pressing the STEP SIZE key After the setting dial is turned clockwise or counte...

Страница 30: ...ld down the specimen receptacle moves quickly upwards into the desired position After the UP key has been released the current position of the specimen receptacle appears on the m display If the speci...

Страница 31: ...an 0 5 mm is set or the user reverses the beginning and end the last entered value is accepted and the previous value is set to the maximum value The cutting window is not saved when the instrument is...

Страница 32: ...ctioning process takes place Pressing the RUN STOP key a second time stops the sectioning process that is in progress The blade moves to the 1st cutting window edge and stays there The m display is th...

Страница 33: ...matic sectioning mode If the AUTO FEED key is pressed one feed motion takes place according to the value programmed in automatic mode If the key is pressed more than once several feed motions take pla...

Страница 34: ...otary knob 2 The feed rate FOR REV for the Blade forward and the Blade back keys can be set between 1 and 5 mm s in 0 5 mm s increments with rotary knob 1 ditto 1 2 Rotary knob 2 Switches the vibratio...

Страница 35: ...r Note The ice tray can be placed on the stage separately for preparation Push the buffer tray Fig 12 5 in as far as it will go small pins Fig 11 4 guide it at the side and front It is held in place b...

Страница 36: ...using the double walled buffer tray the recirculating cooler chiller must be installed PRIOR TO working with specimens Fig 14 8 9 9 Connect hoses Fig 14 8 in the scope of delivery of the double walle...

Страница 37: ...1 Standard scope of delivery for the Leica VT1200 S Screw the manipulator Fig 15 10 onto the specimen plate place it in the buffer tray and adjust it to the desired position The specimen plate is hel...

Страница 38: ...the blade holder through the hole Fig 16 12 and rotate it 45 clockwise Turning the screw Fig 16 13 counterclockwise releases the blade holder allowing it to be replaced The installation is carried ou...

Страница 39: ...he blade as follows 1 Insert the provided size 3 Allen key from the top through the opening Fig 18 14 into the blade holder Fig 18 11 and open the blade holder BH 2 Hold the entire razor blade Fig 18...

Страница 40: ...ng is 18 Fig 19 16 5 5 Routine daily maintenance and switching off the instrument Leica VT1200 Leica VT1200 S After all daily procedures have been finished perform the following Switch off the power s...

Страница 41: ...the lowest position using the DOWN key 1 The user installs the VibroCheck Push the VC along the dovetail guide Fig 21 4 to behind the mark on the baseplate of the instrument rear stop and clamp it do...

Страница 42: ...ion see Fig 23 9 If the value is 0 no improvement of the height amplitude is possible 5 Press the STOP key Using a size 3 Allen key unscrew the clamping screw Fig 23 6 only slightly pull off the cap F...

Страница 43: ...for VT Fig 25 4 5 Prior to assembly the dovetail guide Fig 25 4 is brought down to the lowest position using the DOWN key 1 The user installs the VibroCheck Push the VC along the dovetail guide Fig 25...

Страница 44: ...N STOP is yellow the blade starts to vibrate Fig 28 1 2 Fig 29 6 7 8 8 9 The deviation of the height amplitude is shown on the display in m Fig 28 1 This value can be either positive or negative A num...

Страница 45: ...pted press DOWN flashes green VC moves to lowest position blade is moved to the rear Control panel of the VT shows VIBRO END Fig 30 The LED on the VC is again illuminated in red Fig 30 8 The instrumen...

Страница 46: ...not swing into place inform service Error 22 Timeout while initializing the x axis Timeout Inform service Error 23 DC motor of x axis does not rotate during initialization or in normal operation Infor...

Страница 47: ...on again using the power switch InF 41 Control board C1 back panel and keyboard C2 have different software versions It is possible that due to different software versions some or all functions of the...

Страница 48: ...out or not installed Emitter diode or receiver dirty Clean VibroCheck is defective Leica VT1200 drives to the lowest Z position using the stepper motor after an unsuccessful search Allows blade insta...

Страница 49: ...ction a short beep sounds Note If the 1000 hour limit is exceeded while the interment is in normal operating mode a flashing SEr appears in the upper 3 digit LED for approx three seconds after the sec...

Страница 50: ...side of the instrument above the power switch To do so push an appropriate tool small screwdriver into the slots on the left and right Fig 31 and carefully pull it out Remove the defective fuse and re...

Страница 51: ...cover on the magnifier Remove the blade from the blade holder and dispose of it safely Pull the ice tray and buffer tray off of the dovetail guide and place them on the stage Remove and empty out the...

Страница 52: ...0481 43399 Specimen disc orienting 14 0481 42068 Blade Sapphire blade knife angle of 22 14 0216 39372 Blade holder assembly 14 0481 42030 VibroCheck 14 0481 42075 Display Microscope assembly 14 0481...

Страница 53: ...astic Buffer tray and lid Magnetic holder for the specimen plate Specimen plate non directional Clamp 2x for holding the hose in position in the buffer tray Volume 125 cm3 Autoclavable Order No 14 048...

Страница 54: ...Volume 400 cm3 Non autoclavable Hose set for connecting a recirculating cooler chiller e g 14 0481 48436 Order No 14 0481 44837 Fig 38 Lid for covering the buffer tray The buffer tray is available in...

Страница 55: ...399 Fig 41 Magnet on the underside 2 5 5 Specimen disc orienting Inc swivel handle Inc marks for angling specimen 2 5 and 5 Order No 14 0481 42068 9 1 3 VibroCheck Fig 42 Optional measuring instrument...

Страница 56: ...t S9E Stereozoom microscope 2 eyepieces 10x23 B adjustable Protective objective glass Bearing cylinder with 3 screws Dust cover large Adapter for module LED Hi Power spots 2 arm slot Cover for the LED...

Страница 57: ...p side of the basic instrument Push the magnifier support or microscope support into the bearing bush as far as it will go Fig 47 4 Adjust the magnifier to the desired inclination angle and screw in t...

Страница 58: ...Ordering Information for Optional Accessories Consumables and Spare Parts 9 Fig 48 5 5 6 7 7 9 1 8 Fiber optic illumination cold light source Fig 49 Note Before use carefully read the separately prov...

Страница 59: ...1000 Serves as a light source for the module LED Hi Power spots 2 arm Order No 14 6000 04825 Warning The LED illumination must be operated at the power supply voltage specified on the nameplate on th...

Страница 60: ...n the Leica VT1000 S and Leica VT1200 Leica VT1200 S Selectable temperature range 20 C to 40 C Recommended cooling medium Antifrogen N 14 0481 45443 Mixture with water 50 50 Application example If at...

Страница 61: ...eement The warranty terms of your Leica sales organization or the organization from which you have purchased the contractual product shall apply exclusively Service information If you are in need of t...

Страница 62: ...ww LeicaBiosystems com within the product menu This template has to be used for gathering all required data When returning a product a copy of the filled and signed confirmation has to be enclosed or...

Страница 63: ......

Страница 64: ...LeicaBiosystems com Version 1 8 Revision L 08 2022 Leica Biosystems Nussloch GmbH Heidelberger Strasse 17 19 69226 Nussloch Germany Tel 49 0 6224 143 0 Fax 49 0 6224 143 268 Web www LeicaBiosystems c...

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