Leica TCS SP8 SMD, Руководство пользователя

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SMD Components
9.1.3 Application area FCS/FCCS, FLIM, FLCS, gated FCS
9.1.3.1 Special SMD Hardware
Table 6: Special SMD hardware for FCS/FCCS, FLIM, FLCS, gated FCS
9.1.3.2 Software License
Table 7: Software license for FCS/FCCS, FLIM, FLCS, gated FCS
9.2 Beam Path
The fluorescence necessary for SMD measurement is excited by means of lasers, which
are coupled into the scan head. For FCS and FCCS, continuous wave (cw) lasers are
normally used. However, pulsed lasers can also be used. For FLIM, FLCS or gated FCS, only
pulsed lasers can be used.
The excitation light reaches the specimen through the scan head. The light is collected by
the objective and passes through the scan head to the SMD detectors that can be either
within the spectral module in the scan head or outside the scan head.
For SMD, special detectors are needed that work in single photon counting mode and have
a low background signal. For FLIM, additionally, a high time resolution is necessary,
whereas for FCS a high quantum efficiency is crucial. Therefore, different detectors can be
used, depending on the field of application (see
Table 1 , Table 4 and Table 6 ).
When confocal, external detectors are used, light is split off to optical fibers at the external
interface X1 ("X1 port") with the aid of the X1 adapter, then guided to the photodetectors via
multimode waveguides (
Figure 17
). These waveguide fibers enable the scan head to be
mechanically decoupled from the detectors. If two external detectors are used, light is split
by a beam splitter into the two detection channels. The beam splitter is situated in the SMD
filter cube that is placed in the X1 port adapter on the X1 port.
Pulsed laser laser
coupling unit (LCU) + coupling
as with MP-FLIM
Detectors for SMD
External detection:
PDM APD from MPD ("MPD") + DSN102
And/or internal detection:
SP FLIM photomultiplier (within spectral module, used
for spectral FLIM)
Counting unit TCSPC unit PH300 + Router PHR800
Leica LAS AF FCS FLIM Wizard
PQ SPT SPT1+2