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217
Piezo Focus on an Upright Microscope
22 Piezo Focus on Upright Microscope
If there is a piezo focus installed on your system (see Figure 185 ) pay careful attention to the
following notes:
• Make sure that the specimen carrier is not against the objective and cannot be damaged
by it or cause broken glass. The objective could likewise be damaged.
• Before switching the system on or launching the LAS AF software, ensure that there is
no slide or specimen on the stage and that the specimen stage is in its lowest possible
position. If this is not observed, specimens and objectives can be damaged or destroyed
upon system/software startup by the initialization of the piezo focus.
• Do not carry out the objective change automatically. The automatic motion may damage
the cable of the piezo focus.
• Do not make any adjustments to the piezo focus controller (see
Figure 186
), as it has
already been optimally set up by Leica Service.
• When replacing the objective on the piezo focus, you must perform a teach-in for the
new objective in LAS. Please see the instructions on this topic in the microscope
operating manual.
• Please note that the focus position of an objective with piezo focus is 13 mm lower than
those without piezo focus. In order to guarantee the same focal plane, a spacer is
installed on all other objectives (see
Figure 187 ).
Figure 185: Piezo focus on objective nosepiece
The objective can be moved by 150 μ m in either direction. The total travel is 300 μ m.
Piezo focus controller display:
• Highest position: 350 μ m
• Middle position: 200 μ m
• Lowest position: 50 μ m
• xz scan range: 250 μ m