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F(L)CS Data Acquisition
Figure 166: Settings for acquiring a reference image using photomultipliers
4. For a better comparison of the confocal image with the FCS measurement data, set the
spectral detection window for image acquisition using the internal photomultipliers
corresponding to the transmission of the SMD filter block used in the X1 port adapter.
The laser lines and intensities for image acquisition may be the same as those for the FCS
measurement, but they do not have to be.
5. Check that the external port X1 is switched to the
Mirror
position (
Figure 166, item 3
).
6. Start a continuous scan by clicking the Live image acquisition button ( Figure 166, item 4 )
and start an image acquisition by clicking the
Capture Image image acquisition button
(Figure 166, item 5 ).
18.1.6.2 Image Acquisition with PE APDs (AQR Type) or MPD APDs (PDM Type)
You can also acquire the image directly using the APDs. This procedure is recommended
specifically for specimens with a low signal.
General recommendations for APD image acquisition:
• Slow scan speed (e.g. 10 to 100 Hz)
• Data accumulation (e.g. 4 to 8 frames)
• Best detection range for PE APDs: over 500 to approx. 800 nm, for MPD APDs:
between 430 nm and 700 nm
• Adapt the scale of the color look-up table to the counting rate if necessary