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FLIM Data Acquisition
2. Click the upper radio button (
Figure 126, item 1
).
3. Filter selection:
• For automatic filter selection: Select the
Auto Select
(
Figure 126, item 3
) check box.
Individual filters now cannot be manually selected.
• For manual filter selection: Remove the check in the
Auto Select
check box. From the
filter list, select the suitable filter.
16.2.3.2 Setting for External FLIM or Intensity Image Acquisition
For external FLIM detection or normal intensity image acquisition, it is possible to use the
Empty position ( Figure 126, item 2 ) as needed in the Fluorifier Disc Settings . Remove the
check in the Auto Select check box and select Empty in both menus.
Alternatively, if the effect of polarization is to be investigated, the lower radio button can be
clicked (
Figure 126, item 4
) and the polarization direction defined by moving the slider
position.
16.2.3.3 Setting for FLIM White Light Laser
If the white light laser is used as an excitation source, it is recommended - independent of
the detectors used - to use the filters of the fluorifier disc and, thereby, to select the
corresponding laser wavelengths (usually 488, 514, 561, 594, and 633 nm,
Figure 127 ).
Figure 127: Selecting the laser lines for FLIM white light lasers
Selection switches to set the laser wavelength and the filter wheel can be found in the
Setup FLIM
and
Measurements
steps on the
Acquisition tab (Figure 128
).
o
Figure 128: Selection switches to set the laser wavelength and the filter wheel when using the white light
laser